BGI
Sample Submission and Shipping Guidelines
DNA SAMPLE REQUIREMENT GENERAL GUIDELINES
Notes: For questions on submitting other sample types, or for custom sequencing solutions, please contact our Project Management teams or your BGI Sales Representative to discuss further.
Contact Support:
info@bgi-international.com
1. For HISEQ Platform Based Services
Whole Genome Resequencing ≤800bp normal library (human/ plant/animal/ Fungal/ Bacterial/ Metagenomic)
  • Sample type: genomic DNA/PCR product
  • Quality requirements: >1.5µg, >20ng/µl
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
De novo Genome sequencing ≤800bp normal library(human/ plant/animal/ Fungal/ Bacterial/ Metagenomic)
  • Sample type: genomic DNA/PCR product
  • Quality requirements: >1.5µg, >20ng/µl
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
De novo Genome sequencing 2kb≤X≤6kb(human/ plant/animal/ Fungal/ Bacterial/ Metagenomic)
  • Sample type: genomic DNA/PCR product
  • Quality requirements: m>20µg, c>110ng/µl, no degradation or slight degradation
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
De novo Genome sequencing 8kb≤X≤10kb (human/ plant/animal/ Fungal/ Bacterial/ Metagenomic)
  • Sample type: genomic DNA
  • Quality requirements: m>30µg, c>110ng/µl, no degradation or slight degradation
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
De novo Genome sequencing 20kb (human/ plant/animal/ Fungal/ Bacterial/ Metagenomic)
  • Sample type: genomic DNA
  • Quality requirements: m>40µg, c>110ng/µl, no degradation or slight degradation
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
De novo Genome sequencing 40kb (human/ plant/animal/ Fungal/ Bacterial/ Metagenomic)
  • Sample type: genomic DNA
  • Quality requirements: m>50µg, c>110ng/µl, no degradation or slight degradation
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
De novo Genome sequencing ≤800bp PCR free library (human/ plant/animal/ Fungal/ Bacterial/ Metagenomic)
  • Sample type: genomic DNA
  • Quality requirements: m>10µg, c>30ng/µl, no degradation or slight degradation
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
Whole Exome Sequencing (human/ Animal)

A. Genomic DNA

  • Quality requirements: m>1µg, c>12.5ng/µl, no degradation or slight degradation
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.

B. FFPE Genomic DNA

  • Quality requirements: m>1µg, c>12.5ng/µl, main fragment size ≥250bp
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
Whole Exome Sequencing (Plant)
  • Sample type: genomic DNA
  • Quality requirements: m>10µg, c>30ng/µl, no degradation or slightl degradation
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
Target Region Sequencing

A. Genomic DNA

  • Quality requirements: m>1µg, c>12.5ng/µl, no degradation or slight degradation
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.

B. FFPE Genomic DNA

  • Quality requirements: m>1µg, c>12.5ng/µl, main fragment size ≥250bp
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
ChIP Sequencing
  • Sample type: ChIP-ed DNA
  • Quality requirements: m>10ng, c>1ng/µl, Major Peak in 100bp-500bp(Agilent 2100)
  • Sample volume for QC: 1ul for Qubit, 2ul for Agilent 2100
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
RAD- Sequencing
  • Sample type: genomic DNA
  • Quality requirements: m>300ng, c>10ng/µl, no degradation or slight degradation, No RNA and Protein contamination.
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
Genotyping by Sequencing
  • Sample type: genomic DNA
  • Quality requirements: m>1µg, c>20ng/µl, no degradation or slightly degradation, No RNA and Protein contamination.
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
Target region Bisulfite Sequencing (TBS)
  • Sample type: genomic DNA
  • Quality requirements: m>3µg, c>37.5ng/µl, no degradation or slightly degradation, No RNA and Protein contamination.
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
Whole Genome Bisulfite Sequencing
  • Sample type: genomic DNA
  • Quality requirements: m>1µg, c>15ng/µl, no degradation or slight degradation
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
Meta rDNA Amplicon Sequencing
  • Sample type: genomic DNA
  • Quality requirements: m>1µg, c>20ng/µl, no degradation or slight degradation
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
2. For BIGSEQ-500 Platform Based Services
ChIP Sequencing
  • Sample type: ChIP-ed DNA
  • Quality requirements: m>10ng, c>1ng/µl, Major Peak in 100bp-500bp(Agilent 2100)
  • Sample volume for QC: 1ul for Qubit, 2ul for Agilent 2100
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
Whole Exome Sequencing
  • Sample type: genomic DNA
  • Quality requirements: m>1µg, c>12.5ng/µl, No degradation or slight degradation
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
Whole Genome Sequencing
  • Sample type: genomic DNA
  • Quality requirements: m>1µg, c>12.5ng/µl, No degradation or slight degradation
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis Re-suspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
3. For PACBIO Platform Based Services
Pacbio DNA Sequencing(20Kb)
  • Sample type: genomic DNA
  • Quality requirements: m>10.2ug, c>80ng/µl, no degradation or slight degradation
  • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
Pacbio DNA Sequencing(10Kb)
  • Sample type: genomic DNA
  • Quality requirements: m>10.2µg, c>40ng/µl, No degradation or slight degradation
  • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
Pacbio DNA Sequencing(5Kb)
  • Sample type: genomic DNA
  • Quality requirements: m>4.2µg, c>50ng/µl, No degradation or slight degradation
  • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
Pacbio DNA Sequencing(2Kb)
  • Sample type: genomic DNA
  • Quality requirements: m>1.7µg, c>50ng/µl, No degradation or slight degradation
  • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
Pacbio DNA Sequencing(1Kb)
  • Sample type: genomic DNA
  • Quality requirements: m>1.2µg, c>40ng/µl, no degradation or slight degradation
  • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
Pacbio DNA Sequencing(200bp/500bp)
  • Sample type: genomic DNA
  • Quality requirements: m>0.52µg, c>20ng/µl, no degradation or slight degradation
  • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5
  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
  • Shipment: Dry ice.
RNA SAMPLE REQUIRMENT GUIDELINES
Notes: For questions on submitting other sample types, or for custom sequencing solutions, please contact our Project Management teams or your BGI Sales Representative to discuss further.
Contact Support:
info@bgi-international.com
1. For HISEQ Platform Based Services
Standard/Strand-Specific Transcriptome Sequencing (Human/ Mouse)

A. Total RNA

  • Quality requirements: m>200ng, c>20ng/µl, RIN≥7.0 (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB
  • Shipment: Dry ice.

B. FFPE RNA

  • Quality requirements: m>200ng, c>70ng/µl, DV200≥30%, RIN≥2.0 (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB
  • Shipment: Dry ice.
Standard/Strand-Specific Transcriptome Sequencing (Animals/Plants/Microorganisms)
  • Sample type: Total RNA
  • Quality requirements: m>1ug, c>20ng/µl, RIN≥7.0, 28s/18s≥ 1.0(23s/16s≥ 1.0) (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB
  • Shipment: Dry ice.
RNA-Seq (Quantification) (Human/ Mouse)
  • Sample type: Total RNA
  • Quality requirements: m>2ug, c>80ng/µl, RIN≥7.0, 28s/18s≥ 1.0 (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB.
  • Shipment: Dry ice.
RNA-Seq (Quantification) (Animals/Plants/Microorganisms)
  • Sample type: Total RNA
  • Quality requirements: m>5ug, c>200ng/µl, RIN≥7.0, 28s/18s≥ 1.0 (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB.
  • Shipment: Dry ice.
RNA-Seq (Low-input Quantification)
  • Sample type: Total RNA
  • Quality requirements: m>200ng, c>20ng/µl, RIN≥7.0, 28s/18s≥ 1.0 (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB.
  • Shipment: Dry ice.
Lnc RNA Sequencing (Human/ Mouse)
  • Sample type: Total RNA
  • Quality requirements: m>200ng, c>20ng/µl, RIN≥7.0, 28s/18s≥ 1.0 (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB.
  • Shipment: Dry ice.
Lnc RNA Sequencing (Animals/Plants/Microorganisms)
  • Sample type: Total RNA
  • Quality requirements: m>1ug, c>20ng/µl, RIN≥7.0, 28s/18s≥ 1.0 (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB.
  • Shipment: Dry ice.
Small RNA Sequencing

A. Total RNA

  • Quality requirements: m>5ug, c>200ng/µl, RIN≥8.0, 28s/18s≥1.5(23s/16s≥1.5) (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB
  • Shipment: Dry ice.

B. Small RNA(<200nt)

  • Quality requirements: m>1ug, c>20ng/µl, (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB
  • Shipment: Dry ice.
Low-input Small RNA Sequencing

A. Total RNA

  • Quality requirements: m>200g, c>15ng/µl, RIN≥8.0, 28s/18s≥1.5(23s/16s≥1.5) (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB
  • Shipment: Dry ice.

B. Small RNA(<200nt)

  • Quality requirements: m>100ng, c>5ng/µl (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB
  • Shipment: Dry ice.
2. For BGISEQ Platform Based Services
RNA-Seq (Quantification) (Human/ Mouse)
  • Sample type: Total RNA
  • Quality requirements: m>1ug, c>20ng/µl, RIN≥7.0, 28s/18s≥ 1.0 (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB.
  • Shipment: Dry ice.
RNA-Seq (Quantification) (Animals/Plants/Microorganisms)
  • Sample type: Total RNA
  • Quality requirements: m>2.5ug, c>50ng/µl, RIN≥7.0, 28s/18s≥ 1.0 (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB.
  • Shipment: Dry ice.
RNA-Seq (Low-input Quantification) (Human/ Mouse)
  • Sample type: Total RNA
  • Quality requirements: m>200ng, c>5ng/µl, RIN≥7.0, 28s/18s≥ 1.0 (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB.
  • Shipment: Dry ice.
Small RNA Sequencing

A. Total RNA

  • Quality requirements: m>1ug, c>50ng/µl, RIN≥8.0, 28s/18s≥1.5(23s/16s≥1.5) (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB
  • Shipment: Dry ice.

B. Small RNA(<200nt)

  • Quality requirements: m>400ng, c>10ng/µl, (Agilent2100 or equivalent)
  • Sample volume for QC: 2~3ul
  • Resuspension Buffer: Nuclease-free water, EB
  • Shipment: Dry ice.
EXTRACTION REQUIREMENTS
All extractions are performed to the best of BGI’s ability. The customer is charged for work performed regardless of outcome unless otherwise stated. Additional charges for procurement of kits may apply for select extractions.
FFPE samples (DNA and RNA)
  • Recommended Quantity: DNA≥10 (RNA≥5) FFPE slides
  • Thickness: 5µm-10 µm
  • Diameter: 15 mm×20mm
  • Shipment: submitted at room temperature/blue ice
Cell pellets
  • Recommended Quantity: 106 cells
  • BGI does not provide cell culturing
  • Shipment: submitted on dry ice
Fresh Frozen Tissue
  • Recommended Quantity (DNA Extraction): ≥300mg (for DNA Insert >2kb, Recommended Quantity ≥1g)
  • Recommended Quantity (RNA Extraction): ≥30mg
  • Shipment: submitted on dry ice
Whole Blood
  • Recommended Volume (DNA Extraction): ≥1 mL (EDTA Tube)
  • Recommended Volume (RNA Extraction): 2.5 mL (whole blood, PAXgene Blood RNA Tube)
  • Shipment: Fresh submitted on Blue Ice, Frozen submitted on Dry Ice.
Plasma/Serum
  • Recommended Volume (DNA Extraction): ≥5 mL
  • Recommended Volume (RNA Extraction): ≥1 mL
  • Shipment: Dry Ice
Stool
  • Recommended Quantity: 50~100 mg (Solid)
  • Recommended Volume: 500uL (OMNIgene•GUT(OMR-200), DNA Genotek Collection tube)
  • Shipment: Solid submitted on dry ice, Collection tube submitted at room temperature/blue ice.
Soil
  • Recommended Quantity: 50~150mg
  • Shipment: submitted on dry ice.
Swab
  • Recommended Quantity: 2 tubes/sample, 1 swap/tube
  • Shipment: submitted on dry ice.
BGI SAMPLE QUALITY CONTROL ASSESSMENT
BGI performs QC free of charge.
DNA RNA Pre-Made Libraries
Agilent 2100 BioAnalyzer

Microplate Reader/Qubit

Gel Electrophoresis

Resources
Tell Us About Your Project Return To The Top
首页