Single Cell Sequencing
Sequencing Services
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  • Service Description

    Single cell sequencing can facilitate the elucidation of cell lineage relationships. The major applications of this technique include profiling scarce clinical samples (i.e. circulating tumor cells), pre-implantation genetic diagnosis, embryonic development research, and tumor progression analysis.

  • Sequencing Service Specification

    Sequencing services are performed on the HiSeq 4000 platform.

    Advancing the possibilities of single cell sequencing in human disease research, BGI has developed an innovative end-to-end solution for genomics analysis at the single cell level. Within this solution, multiple displacement amplification (MDA) has been further enhanced and incorporated into BGI’s whole genome amplification (WGA) protocol, which enables uniform amplification of genomic DNA/RNA from single cells with negligible sequence bias and maximized genome coverage.

    Sample Requirements
    Sample Requirements
    • Fresh Tissue: A size of 1-2 cm3 is recommended. Tissue samples should be immediately stored in liquid nitrogen or at -80 ℃ after surgical resection, without other solvents treatment.
    • Whole Blood (or Bone Marrow): The total volume should be no less than 5 ml. Samples should be collected with anticoagulant tube and stored at -80℃.
    • Cell Suspensions: No fewer than 100,000 cells are recommended. It is necessary to follow the standard cell cryopreservation operation protocols, freeze cells gradually with cryopreservation media, and store them in liquid nitrogen or at -80℃.
    • Isolated Single Cells: Single cells should be stored separately in 3-5 μL solvent (e.g., PBS), in DNase/Rnase free PCR tube (200 μL), and stored at -80 ℃ for no longer than one week. Cells should be free of nucleic acid–binding dyes.
    Data Analysis
    Standard Bioinformatics Analysis
    • Data filtering (removing adaptors, contamination, and low-quality reads from raw reads)
    • Alignment and summary of data production
    • SNP calling, annotation, and statistics
    • InDel calling, annotation, and statistics
    • CNV calling, annotation, and statistics (only for whole genome resequencing)
    • SV calling, annotation, and statistics (only for whole genome resequencing)

    Customized bioinformatics options are available.