Sequencing Services
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  • Service Description

    ChIP-Seq, also known as ChIP-Sequencing, is widely used to analyze protein interactions with DNA. It combines chromatin immunoprecipitation (ChIP) with next generation sequencing (NGS) to identify binding sites of DNA-associated proteins, and can be used to precisely map global binding sites for any protein of interest.

    The application of NGS to ChIP has revealed insights into gene regulation events that play a role in various diseases, such as cancer.

    ChIP sequencing offers higher resolution and more precise and abundant information in comparison with array-based methods.

    • Wide detection range: genome wide protein DNA interaction studies
    • Cost-effective: less data required for identifying the binding sites in whole genome
    • Only require low input DNA required: As low as 5ng ChIP-ed DNA is adequate
  • Sequencing Service Specification

    50SE,100PE or 150PE

    Services Available on either the BGISEQ-500 or HiSeq platforms.

    Data Analysis
    • Data Analysis
      • Data filtering (removing adaptor sequences, contamination and low-quality reads from raw reads)
      • Reads Alignment
      • Genome-wide distribution of ChIP sequencing reads
      • Genome-wide peak scanning and distribution
      • GO function analysis of peak-related genes
      • Difference analysis of multi-samples
      • Tag detection near transcription start sites
      • UCSC Genome Browser instruction
      • ChIP sequencing reads distribution around transcription start sites Custom Bioinformatics Analysis
      • We can also perform customized analysis to meet specific needs of your projects.
    Sample Requirements
    Sample Requirements
    • ChIP-ed DNA
    • m≥20 ng
    • Main Peak in 100 bp-500 bp