Whole Genome Bisulfite Sequencing
Sequencing Services
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  • Service Description

    Cytosine methylation can significantly modify gene expression and chromatin remodeling. Leveraging the power of next-generation sequencing (NGS), whole-genome bisulfite sequencing (WGBS) or bisulfite sequencing (Bisulfite-Seq or BS-Seq) is a methodology that uses bisulfite treatment combined with high-throughput sequencing to provide insight into methylation patterns at a single nucleotide level.

    WGBS is currently the sole method that can attain both single-base resolution and genome-wide coverage. It has been successfully applied by BGI for elucidation of the methylomes of human cells as well as of many other species such as silkworms, rats, mice, zebra fish, maize and rice.

    Whole-genome bisulfite sequencing is considered the ultimate method for methylcytosine analysis, as the technique allows researchers to:

    • Simultaneously observe the methylation patterns of all CpG, CHG and CHH sites present in the sample of interest
    • Profile DNA methylations in the entire methylome for studies on development, cell differentiation, cell cycle, DNA repair, genomic imprinting
    • Compare differentially methylated loci from different samples, for example, healthy controls versus patients with cancer
    • Derive correlations between cytosine methylation and transcriptional regulation
    • Investigate inherited methylations in nearly any organism
  • Sequencing Service Specification

    Services are performed using the HiSeq 4000 platform, PE150.250~300-bp insert bisulfite treated DNA library

    The multi-step process employs bisulfite conversion, PCR amplification and next-generation sequencing to determine the methylation sites across the methylome.

    Chemical treatment of DNA with sodium bisulfite converts unmethylated cytosines into uracils. The DNA is then subjected to a PCR assay, where all uracils are converted to thymidines. The resulting DNA amplicons are sequenced at high coverage. The obtained sequencing reads are mapped against a reference genome to enable the identification of the methylated loci in the sample.

    Sample Requirements
    Sample Requirements
    • DNA amount: ≥ 5 μg
    • DNA concentration: ≥ 50 ng/μl
    • Purity: OD260/280 ≥ 1.8 ~ 2.0 without degradation or RNA contamination
    Data Analysis

    BGI offers extensive analysis options providing unmatched flexibility in delivering reliable data for achieving various research aims ranging from cancer research to understanding basic cellular processes.