Long Non-coding RNA Sequencing
Sequencing Services
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  • Service Description

    Long non-coding RNAs (lncRNAs) are a large class of transcribed RNA molecules with a length of more than 200 nucleotides that do not encode proteins. lncRNAs are thought to encompass nearly 30,000 different transcripts in humans, hence lncRNA transcripts account for the major part of the non-coding transcriptome.

    lncRNA discovery is still at a an early stage and only a small proportion of lncRNAs have so far been investigated. Although we can start to classify different types of lncRNA functions, we are still far from being able to predict the function of new lncRNAs.

    Sequencing Service Description

    BGI offers expression profiling as one way to uncover the function of lncRNA. Identifying lncRNAs that are differentially expressed during development or in particular situations can shed light on their potential functions. Alternatively, looking for lncRNAs and protein-coding genes whose expression is correlated, can indicate co-regulation or related functions.

    Sequencing is performed on the HiSeq 4000 platform, paired-end 150 bp 250~300 bp insert strand specific library with rRNA removal (Ribo-ZeroTM Magnetic Kit)

  • Sample Requirements
    Sample Requirements
    • Total RNA amount: ≥ 4 μg; RNA concentration: ≥ 50 ng /μl
    • RIN value ≥ 6.3 for plants and fungi; RIN value ≥ 6.8 for animals
    • Purity: OD260/280 ≥ 2.0, OD260/230 ≥ 2.0, without degradation and DNA contamination

    FFPE sample:> 10 scrolls or slides. Samples should be tested and pre-qualified by gel electrophoresis.

    Data Analysis
    Data Analysis
    • Sequencing assessment (alignment statistics)
    • LncRNA identification and function prediction
    • Analysis of differentially expressed
    • LncRNA family prediction and classification
    • Customized bioinformatics analysis options available