RNA (Transcriptome) Sequencing
Sequencing Services
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  • Service Description

    Transcriptome sequencing is used to reveal the presence, quantity and structure of RNA in a biological sample under specific conditions. Compared to hybridization-based RNA quantification method such as microarray, sequencing-based transcriptome detection can quantify gene expression with low background, high accuracy and high levels of reproducibility within a large dynamic range. In addition, transcriptome sequencing does not require existing genome sequence and can detect mutations/SNPs, splice variants and fusion genes that can not be obtained by microarrays.

    BGI Also provides Whole transcriptome sequencing, which provides sequencing information for both mRNA and Long non-coding RNA (lncRNA). Whole Transcriptome Sequencing can identify novel lncRNAs besides detection and quantification of known lncRNAs.

    Project Workflow

    We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

    Sample QC
    Library QC
    Data QC
    Sample
    preparation
    Library
    construction
    Sequencing
    Raw data
    output
    Bioinformatics
    Analysis
  • Sequencing Service Specification

    BGI transcriptome sequencing services are executed with the Illumina HiSeq 4000 sequencing system.

    Sample Preparation and Services
    • Multiple choices for mRNA enrichment and rRNA removal kits
    • Regular and Strand-specific transcriptome library
    • 100 bp and 150bp paired-end sequencing options available
    • Raw data and bioinformatics analysis are available in standard file formats
    • Advanced and custom bioinformatics data analysis
    • Cloud-based data storage and delivery system
    • ≥30 Million reads per sample recommended
    Turn Around Time
    • Typical 40 working days from sample QC acceptance to filtered raw data availability.
    • Expedited services are available, contact your local BGI specialist for details.
    Sample Requirements

    We can process your total RNA, Blood, Cell line, FFPE, Fresh Frozen tissue and Single Cell samples from a variety of species, with the following general requirements:

    Sample type Species Amount Concentration

    (ng/μL)

    RIN value OD
    Total RNA

    Human /Animal

    ≥200ng

    ≥20

    RIN≥7.0

    N/A

    Fungi

    ≥1ug

    ≥40

    RIN≥6.5

    OD260/280≥1.8

    OD260/230≥1.8

    28S/18S≥1.0

    Plant

    ≥200ng

    ≥20

    RIN≥6.0

    Prokaryotes

    ≥1ug

    ≥40

    RIN≥7.0

    Insect

    ≥200ng

    ≥20

    N/A

    N/A

    Data Analysis

    In addition to raw data output, BGI offers a range of standard and customized bioinformatics pipelines for your Transcriptome sequencing project.

    Reports and output data flies are delivered in industry standard file formats: FASTQ, BAM, FPKM calculation and Excel.

    • Standard Analysis
      • Filtering
      • Alignment
      • Quantitative expression profiles
      • Detection of differentially expressed genes
      • Spliced transcript analysis
      • Fusion gene analysis
      • SNP and Indel detection
      • RNA editing analysis
      • Gene ontology analysis
      • Pathway enrichment analysis
      • Hierarchical clustering analysis
      • Additionally, for Whole Transcriptome Sequencing:
      • lncRNA analysis included
    • Customized Analysis
      • Further customization of Bioinformatics analysis to suit your unique
      • project is available:
      • Please contact your BGI technical representative
  • Resources content for this page including relevant research papers we have published, case studies and examples of data output will be available soon. Please contact us for more information regarding any additional requests for information or resources not currently listed on our website.

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