Small RNA Sequencing
Sequencing Services
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  • Service Description

    Small RNAs are a type of non-coding RNA (ncRNA) molecule that are less than 200 nucloetides in length are often involved in post-transcriptional regulation through RNA– RNA interactions.

    Small RNA sequencing first isolate then sequence small RNA species, such as microRNA (miRNA). Small RNA sequencing capture the complete range of small RNA and miRNA species in a sample, and can detect and quantify thousands of small RNA and miRNA sequences with great sensitivity and dynamic range. It has been applied to discover novel miRNAs and other small noncoding RNAs, to examine the differential expression of all small RNAs in and to characeterize variations with single-base resolution.

    Project Workflow

    We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

    Sample QC
    Library QC
    Data QC
    Sample
    preparation
    Library
    construction
    Sequencing
    Raw data
    output
    Bioinformatics
    Analysis
  • Sequencing Service Specification

    BGI small RNA Sequencing services are executed with the Illumina HiSeq4000 and BGISEQ-500 sequencing system.

    Sample preparation and services
    • 50 bp Single End sequencing options available
    • Clean data and advanced bioinformatics analysis are available in standard file formats
    • Standard and custom bioinformatics data analysis
    • Available Data storage
    Sequencing Quality Standard
    • Q20≥90%
    • Adaptor and null rate10%
    • Small RNA tag rate20%
    • Clean reads: no less than quantity the contract needed
    Turn Around Time
    • Typical 30 working days after sample receipt for raw data delivery.
    • Expedited services are available, contact your local BGI specialist for details.
    Sample Requirements

    We can process your sample of plant,animal,insect,Fungi, prokaryote,plasma, etc. with the following general requirements:

    DNA concentration Minimum Sample Volume
    Regular Samples

    Intact genomic DNA ≥ 1µg, Concentration ≥ 15 ng/ µl

    15 µl

    Low input Samples

    Intact genomic DNA ≥ 100 ng, Concentration ≥ 20 ng/ µl

    15 µl

    Data Analysis

    Besides clean data output, BGI offers a range of standard and customized bioinformatics pipelines for your small RNA sequencing project.

    Reports and output data flies are delivered in industry standard file formats: .xls, .png

    • Standard Analysis
      • Filtering
      • Summarize the length distribution of small RNA
      • Analyze common and specific sequences between two samples
      • Explore small RNA distribution across selected genome
      • Identify rRNAs, tRNAs, snRNAs, snoRNAs etc.
      • Identify repeat associated small RNAs
      • Identify small RNA reads which could align to exon/intron
      • Identify known miRNAs by aligning to designated part of miRBase
      • Analyze the expression pattern of known miRNAs
      • Annotate small RNAs into several categories based on priority
      • Predict novel miRNAs and their secondary structures by Mireap and
      • miRDeep from unannotated small RNAs;
      • Family analysis of known miRNAs
    • Available Advanced Analysis
      • Target genes prediction of novel known miRNA and miRNA
      • GO annotation and KEGG pathway analysis of known miRNA and novel miRNA Target genes
      • Base editing analysis of known miRNA
      • Differential expression analysis of known miRNA
      • Differential expression analysis of novel miRNA
      • Target genes prediction of differential miRNA
      • GO annotation and KEGG pathway analysis of differential miRNA Target genes
      • piRNA annotation
      • snoRNA annotation
    • Customized Analysis
      • Further customization of Bioinformatics analysis to suit your unique project is available:
      • Please contact your BGI technical representative
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