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FFPE Samples

FFPE Samples

FFPEFormalin-fixed, paraffin-embedded (FFPE) samples are common biological materials for disease diagnosis and scientific research. Because FFPE sample tissues can be stored indefinitely at room temperature, and nucleic acids (both DNA and RNA) can be recovered after decades from the original fixation, they have become an important resource for historical studies in medicine. It is, however, challenging to get intact information from such samples, as severe degradation, damage, and molecular or biological modification could occur during sample preparation and store process.

In recent years, there were many publications about the discovery of gene variations, gene expression profiling changes, and protein biomarkers with FFPE tissues, which generally adopt immunohistochemical method, Real-time PCR, and array-based detection methods. However, there are quite few usages of next-gen sequencing (NGS) method in deciphering the FFPE nucleotide information. In order to unveil the information concealed in FFPE samples, BGI has worked relentlessly in applying NGS to FFPE samples. We expect that NGS technologies with FFPE samples will substantially facilitate our understanding of undefined pathological mechanisms and broaden our insights in biomedical research.


  • Low input: As low as 200ng as for WGS, WES and RNA-Seq.
  • High throughput: Million of reads available for downstream analysis.
  • FFPE-Omics:Comprehensive information with multiple sequencing technologies.
  • High rRNA removal efficiency: Lower than 10% of the reads mapping on rRNA gene sequences, leading to increased sample throughput
Technical Information

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