Choose   Change

slide-4

Whole Transcriptome Sequencing

Overview

_______________________________________________________
An Accessible, Cost Effective Sequencing Solution to Enable Rapid Discovery with Industry Leading Turnaround Times.


Service Description

Transcriptome Sequencing is used to reveal the presence, quantity and structure of RNA in a biological sample under speci c conditions. Compared to hybridization-based RNA quantification method such as microarray, sequencing-based transcriptome detection can quantify gene expression with low background, high accuracy and high levels of reproducibility within a large dynamic range. In addition, transcriptome sequencing does not require existing genome sequence and can detect mutations/SNPs, splice variants and fusion genes that can not be obtained by microarrays.

Sequencing Service Specification

BGI transcriptome sequencing services are executed with the Illumina HiSeq 4000 sequencing system.

Sequencing Details

  • Multiple choices for mRNA enrichment and rRNA removal kits
  • Regular and Strand-speci c transcriptome library
  • 100 bp and 150bp paired-end sequencing options available
  • Raw data and bioinformatics analysis are available in standard le formats
  • Advanced and custom bioinformatics data analysis
  • Cloud-based data storage and delivery system
  • ≥30 Million reads per sample recommended

Turn Around Time

  • Typical 40 working days from sample QC acceptance to ltered raw data availability
  • Expedited services are available, contact your local BGI specialist for details

Sample Requirements

We can process your total RNA, Blood, Cell line, FFPE, Fresh Frozen tissue and Single Cell samples from a variety of species, with the following general requirements:
_______________________________________________________________________
BGI Transcriptome sequencing sample requirements

Project Workflow and Data Analysis

Project Workflow

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
_________________________________________________________________________

BGI RNA Seq Transcriptome project workflow

Data Analysis

In addition to raw data output, BGI o ers a range of standard and customized bioinformatics pipelines for your Transcriptome sequencing project.

Reports and output data files are delivered in industry standard file formats: FASTQ, BAM, FPKM calculation and Excel.
_________________________________________________________________________

BGI Transcriptome sequencing data analysis

Request a Quote

Contact form for BGI
  •   Whole Genome Resequencing
      Exome Sequencing
      Target Region Sequencing
      de novo Sequencing
      RNA-Seq (Transcriptome)
      RNA-Seq (Quantification)
      Small RNA Sequencing
      Long Non-coding RNA Sequencing
      Whole Genome Bilsulfite Sequencing
      Reduced Representation Bilsulfite Sequencing
      MeDIP Sequencing
      ChIP-Seq
      16S rDNA tagging
      Wole Genome Metagenomic Sequencing
      Microbial Gene Catalog
      Proteomics Services
      Others
  •   Human
      Others
  •   Yes
      No

Contact

Don’t hesitate to contact us to request a quote or to talk more about your requirements and how we can support your needs.


(+45) 80 300 800 (Europe)
(+1) 617 500 2741 (Americas)
(+852) 36103510 (Asia Pacific)
(+86) 755 25273698 (China)



BGI certificates

Copyright © BGI 2016