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Mouse Exome Sequencing

Overview

Our solution utilizes Agilent SureSelect XT Mouse All Exon Kit, whose 50 Mb capture covers the complete mouse exome and spans over 221,784 exons and 24,306 genes. The content was developed based on the current UCSC mm9 mouse genome build. A BED file with the exon and gene annotation is available on the Agilent Array website. Combined with BGI’s powerful next-generation sequencing platform and bioinformatics analysis capability, we have become one of the most important centers for mouse genome research.

Benefits:

    • Highly effective: Designed towards specific species; more effective for capturing the mouse exons
    • Wide detection range: Capture both common and rare variants
    • Highly cost-effective: Focus on ~1% of the genome where most disease- or trait-related variations are located.
Technical Information

BGI adopts Agilent SureSelect XT Mouse All Exon Kit to capture the mouse exome. The enriched exome library passing QC is sequenced by Illumina HiSeq2500, and the output data is analyzed by BGI’s standard bioinformatics pipeline.

Sample Requirements:

For the genomic DNA samples we require:

    • Purity: OD 260/280=1.8~2.0, without degradation and RNA contamination
    • Concentration: ≥30ng/μl
    • Quantity demanded: ≥30μg (using NimbleGen Sequence Capture Array when the targeted region is larger than 17Mb) or ≥6μg (using Agilent SureSelect System or using NimbleGen Sequence Capture Array when the targeted region is smaller than 17Mb).

Cases

We sequenced 7 mouse exomes as a demonstrable evaluation of mouse exome data quality. After enrichment, we sequenced the exomes using HiSeq 2000, generating over 2.5 Gb of clean data for each sample.

Our results suggest that the mouse exome capture platform can capture all exons and generate high quality data that is comparable with human exome sequencing results for reliable SNP detection. Our mouse exome sequencing platform offers a highly efficient and cost-effective way to investigate the mouse genome information.

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  •   Whole Genome Resequencing
      Exome Sequencing
      Target Region Sequencing
      de novo Sequencing
      RNA-Seq (Transcriptome)
      RNA-Seq (Quantification)
      Small RNA Sequencing
      Long Non-coding RNA Sequencing
      Whole Genome Bilsulfite Sequencing
      Reduced Representation Bilsulfite Sequencing
      MeDIP Sequencing
      ChIP-Seq
      16S rDNA tagging
      Wole Genome Metagenomic Sequencing
      Microbial Gene Catalog
      Proteomics Services
      Others
  •   Human
      Others
  •   Yes
      No

Contact Us

Don’t hesitate to contact us to request a quote or to talk more about your requirements and how we can support your needs.

(+45) 80 300 800 (Europe)

(+1) 617 500 2741 (Americas)

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