Choose   Change

picture of BGI laboratory

Bisulfite Sequencing

Overview

Bisulfite Sequencing

Bi-sulfiteBisulfite Sequencing is a methodology that uses bisulfite treatment combined with high-throughput sequencing to draw the most complete picture of a DNA methylome. BS is currently the sole method that can attain both single-base resolution and genome-wide coverage. It has been successfully applied by BGI for elucidation of the methylomes of human cells as well as of other species such as silkworm, rat, mouse, Arabidopsis, zebra fish, maize, rice, bee, chicken, and so on.

Benefits:

  • Single-base resolution: Analyze methylation state of every Cytosine accurately
  • Wide applications: Suitable for all the species with accurate genome map
  • High-quality data: Q20 > 90%, Q30>80%
Technical Information

Workflow:

Bisulfite_Sequencing_Workflow

Bioinformatics:

Standard Bioinformatics Analysis

  1. Data Filtering (removing adaptor sequences, contaimination and low-quality reads from raw reads) and Production Statistics
  2. Reads Alignment
  3. Sequence Depth and Coverage Analysis
  4. Calculation of Methylation Level
  5. Global Trends of Methylome
  6. Genome-wide Methylation Profiling
  7. Identification of Differentially Methylated Regions (DMRs)

Custom Bioinformatics Analysis

  1. We can also perform customized analysis to meet requirements of specific projects.

Sample Requirements:

For the genomic DNA samples you will provide:

  1. Purity: OD 260/280=1.8-2.0, without DNA degradation and protein pollution
  2. DNA amount: single library preparation starts from at least 5μg

Recommended Sequencing Depth:

30 times of the genome size

Turnaround Time:

The standard turnaround time for the workflow, only including standard bioinformatics analysis, is ~40 work days.

Cases

Single Base-Resolution Methylome of the Silkworm Reveals a Sparse Epigenomic Map.Nature Biotechnology. 28:516-520 (2010).

worms

Here we survey the methylome of a model insect, the silkworm Bombyx mori, at single-base resolution using Illumina high-throughput bisulfite sequencing (MethylC-Seq). CG methylation is substantially enriched in gene bodies and is positively correlated with gene expression levels, suggesting it has a positive role in gene transcription. This work contributes to our understanding of epigenetics in insects, and in contrast to previous studies of the highly methylated genomes of Arabidopsis1 and human2, demonstrates a strategy for sequencing the epigenomes of organisms such as insects that have low levels of methylation.

The DNA Methylome of Human Peripheral Blood Mononuclear Cells. PloS Biology. DOI: 10.1371/journal.pbio.1000533 (2010)

redcells

Using whole-genome bisulfite sequencing at 24.7-fold coverage, we report a comprehensive (92.62%) methylome and analysis of the unique sequences in human peripheral blood mononuclear cells (PBMC) from the same Asian individual whose genome was deciphered in the YH project. Analysis of the PBMC methylome revealed a rich epigenomic landscape for 20 distinct genomic features, including regulatory, protein-coding, non-coding, RNA-coding, and repeat sequences. Integration of our methylome data with the YH genome sequence enabled a first comprehensive assessment of allele-specific methylation (ASM) between the two haploid methylomes of any individual and allowed the identification of 599 haploid differentially methylated regions (hDMRs) covering 287 genes.

Request a Quote

Contact form for BGI
  •   Whole Genome Resequencing
      Exome Sequencing
      Target Region Sequencing
      de novo Sequencing
      RNA-Seq (Transcriptome)
      RNA-Seq (Quantification)
      Small RNA Sequencing
      Long Non-coding RNA Sequencing
      Whole Genome Bilsulfite Sequencing
      Reduced Representation Bilsulfite Sequencing
      MeDIP Sequencing
      ChIP-Seq
      16S rDNA tagging
      Wole Genome Metagenomic Sequencing
      Microbial Gene Catalog
      Proteomics Services
      Others
  •   Human
      Others
  •   Yes
      No

Contact USA

Don’t hesitate to contact us to request a quote or to talk more about your requirements and how we can support your needs.


(+45) 80 300 800 (Europe)
(+1) 617 500 2741 (Americas)
(+852) 36103510 (Asia Pacific)
(+86) 755 25273698 (China)



BGI certificates

Copyright © BGI 2016