Turn Around Time
- Typical 40 working days from sample QC acceptance to filtered raw data availability
- Expedited services are available, contact your local BGI specialist for details
BGI has many years expertise in single cell sequencing. Single cell sequencing can facilitate the elucidation of cell lineage relationships. Advancing the possibilities of single cell sequencing in human disease research, BGI has developed innovative end-to-end solutions for genomics analysis at the single cell level. Within the solutions, multiple displacement amplification (MDA) and Smart-seq 2 has been further enhanced and incorporated into BGI’s whole genome/transcriptome amplification protocol, which enable uniform amplification of genomic DNA and full length RNA from single cells with negligible sequence bias and maximized genome coverage.
The major applications of this technique include:
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
BGI's Single Cell Sequencing services are performed on the HiSeq 4000 platform.
Fresh Tissue: A mass of 0.5~1g is recommended. Tissue samples should be immediately stored in liquid nitrogen or at -80 ℃ after surgical resection, without other solvents treatment.
Whole Blood (or Bone Marrow): The total volume should be no less than 5 ml. Samples should be collected with anticoagulant tube and stored at -80℃.
Cell Suspensions: No fewer than 200,000 cells are recommended. It is necessary to follow the standard cell cryopreservation operation protocols, freeze cells gradually with cryopreservation media, and store them in liquid nitrogen or at -80℃.
Isolated Single Cells: Single cells for DNA sequencing should be stored separately in DNase/Rnase free PCR tube (200 μL) with 3-5 μL PBS, while single cells for RNA sequencing should be stored separately in 3-5 μL lysis buffer that provided by BGI. The sample should be stored at -80 ℃ for no longer than one week. Cells should be free of nucleic acid–binding dyes.
In addition to raw data output, BGI offers a range of standard and customized bioinformatics pipelines for your whole genome sequencing project.
Data filtering (removing adaptors, contamination, and low-quality reads from raw reads)
Alignment and summary of data production
SNP calling, annotation, and statistics
InDel calling, annotation, and statistics
CNV calling, annotation, and statistics (only for whole genome resequencing)
SV calling, annotation, and statistics (only for whole genome resequencing)
Data filtering includes removing adaptors contamination – low quality reads from raw reads.
Assessment of sequencing
Gene expression and annotation (Gene coverage and coverage depth)
Gene expression difference analysis
Expression pattern analysis of DEGs
Gene ontology analysis of DEGs
Pathway enrichment analysis of DEGs
Refinement of gene structures
Identification of alternative spliced transcripts
Further customization of Bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative