RNA-Seq for Quantification

RNA-Seq for Quantification

Service Description

BGI’s RNA-Seq (Quantification) is used to reveal the presence and quantity of RNA in a biological sample under specific conditions. Compared to hybridization-based RNA quantification methods such as microarray analysis, sequencing-based transcriptome detection can quantify gene expression with low background, high accuracy and high levels of reproducibility within a large dynamic range. RNA-Seq (Quantification) allows the entire transcriptome to be surveyed in a very high-throughput and quantitative manner at single-base resolution.

Project Workflow

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

  • Sample QC
  • Library QC
  • Sequencing QC
  • Data QC
  • Sample Preparation
  • Library Preparation
  • Sequencing
  • Raw Data Output
  • Bioinformatics Analysis

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Sequencing Service Specification

BGI RNA-Seq (Quantification) Sequencing services can be executed with our proprietary nique DNBseq™ technology platform with the benefit of lower cost and turn-around-time.

  • Sample Preparation and Services

    Sample Preparation and Services

    • 50bp single end sequencing
    • Raw data and bioinformatics analysis are available in standard file formats
    • Advanced and custom bioinformatics data analysis
    • Cloud-based data storage and delivery system
    • ≥20 Million reads per sample recommended
  • Turn Around Time

    Turn Around Time

    • DNBseq™ Platform: Typical 25 working days from sample QC acceptance to filtered raw data availability
    • Expedited services are available, contact your local BGI specialist for details

Sample Requirements

We can process your total RNA, blood, cell line, FFPE, fresh frozen tissue and single cell samples from a variety of species, with the following general requirements:

RNA Amount and Concentration

Minimum Sample Volume

Regular Samples

m ≥ 1μg,concentration ≥ 20 ng/ μl

15 μl

Low Input Samples

m ≥ 0.2μg,concentration ≥ 20 ng/ μl

15 μl

Data Analysis

In addition to clean data output, BGI offers a range of standard and customized bioinformatics pipelines for your RNA-Seq (Quantitation) Gene Expression Sequencing project.
Reports and output data files are delivered in industry standard FASTQ, BAM. Excel file formats with publication-ready tables and figures.

Standard Analysis

  • filtering

  • Assessment of sequencing statistics

  • Gene Expression annotation

  • Differential gene expression analysis

  • Identification of differentially expressed genes in sample groups

  • Expression pattern analysis

  • Gene Ontology enrichment analysis

  • Pathway enrichment analysis

  • Protein-protein interaction network analysis

  • Transcription factor analysis (for plants)

  • PCA analysis

  • Condition specific expressed analysis

  • Gene Co-expression Network Analysis

Customized analysis

  • Further customization of Bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative

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