Small RNAs are a type of non-coding RNA (ncRNA) molecules that are less than 200nt in length. They are often involved in gene silencing and post- transcriptional regulation of gene expression. Small RNA sequencing is used to discover novel small RNAs, examine the dierential expression of all small RNAs and to characterize variations with single-base resolution.
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
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Sequencing Service Specification
BGI Small RNA Sequencing services are executed with BGI's DNBseq™ Technology Platform.
Sample Preparation and Services
50bp single-end sequencing reads
Standard output 20 Million reads per sample
Clean data and bioinformatics analysis are available in standard file formats
Standard and customized bioinformatics data analysis are available
Cloud-based data storage and delivery system
Sequencing Quality Standard
Adaptor and null rate 10%
Small RNA tag rate 20%
Clean reads: no less than quantity the contract needed
Turn Around Time
Typical 30 working days from sample QC acceptance to data delivery
Expedited services are available, contact your local BGI specialist for details
We can process your sample of human, plant, animal, and microbial samples, with the following general requirements:
AMOUNT AND CONCENTRATION
Total RNA Samples
Total RNA≥1μg, Concentration≥15 ng/μl
In addition to clean data output, BGI offers a range of standard and customized bioinformatics pipelines for your small RNA sequencing project.
Reports and output data files are delivered in industry standard FASTQ, and Excel file formats with publication-ready tables and figures.
STANDARD BIOINFORMATICS ANALYSIS
Length distribution of small RNAs
Analysis of common and specific sequences between two samples
Small RNAs distribution across selected genome
Identification of rRNAs, tRNAs, snRNAs, snoRNAs, etc.
Identification of repeat associated small RNAs
Identification of small RNA sequences which could align to exon/intron
Identification of known miRNAs by aligning to designated part of miRBase
Analysis of the expression pattern of known miRNAs
Classification of small RNAs into several categories based on customer preference
Prediction of novel miRNAs and their secondary structures by Mireap and miRDeep from unannotated small RNAs
Family analysis of known miRNAs
Further customization of Bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative