Isobaric Label

Service Description

We have developed innovative solutions for optimizing the process of accurately quantifying proteins in complex biological matrices and other complex mixtures.

 

Isobaric Label TMT Quantitation Proteomics

Isobaric Label services incorporate sample multiplexing to provide deep proteome coverage and highly precise quantitation of small or medium-sized sample sets. Samples are prepared and labeled separately, then all samples are pooled and analyzed by nano-flow LC-MS/MS1.

Project Workflow








Protein extraction

Protein digestion

Isobaric labeling

HPLC fractionation

LC-MS/MS (DDA)

Data analysis

Reference

[1] Wong, J. W. H., & Cagney, G. (2009). An Overview of Label-Free Quantitation Methods in Proteomics by Mass Spectrometry. Proteome Bioinformatics, 273–283.doi:10.1007/978-1-60761-444-9_18.

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Mass Spectrometry Service Specification

Isobaric Label Quantitative Proteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF/HF-X).

  • Sample Preparation and Services

    Sample Preparation and Services

    • The QC of enzymatic hydrolysis and labeling steps are strictly controlled by LC-MS DDA analysis
    • 1h/fraction; 20 fractions for eukaryotes;10 fractions for prokaryotes
  • Quality Standard

    Quality Standard

    • Summary includes all methods and data analysis
    • Reports provided in Excel or PDF format, RAW files available upon request
  • Turn Around Time

    Turn Around Time

    • Typical 4-5 weeks from sample QC acceptance to data report delivery

Sample type

Sample type

Recommend Amount

Animal

Common animal tissues:
animal internal organs (heart, liver, spleen, lung, kidney), skin, muscle, brain, etc

≥ 10 mg

Animal

Mollusks
(Toxoplasma, Schistosomiasis, Drosophila, Acarid, Plutella xylostella, Laodelphax, Cestode, Cicada, Hematodinium, etc.)

≥ 10 mg

Cell

Suspended cells, adherent cells

≥ 1×10^7

Cell

Cell culture supernatant

≥ 10 mL

Exosome

Exosome isolated by customer

≥ 50 µg, ≥ 0.5 µg/µL

Fluid

Plasma, serum (remove highly-abundant protein)

≥ 200 µL

Fluid

Plasma, serum (with highly-abundant protein)

≥ 10 µL

Fluid

Amniotic fluid, cerebrospinal fluid, semen, etc. (remove highly-abundant protein)

≥ 1 mL

Fluid

Amniotic fluid, cerebrospinal fluid, semen, etc. (with highly-abundant protein)

≥ 200 µL

Fluid

Saliva, milk

≥ 200 µL

Fluid

Urine

≥ 30 mL

Plant

Tear

≥ 15 µL

Plant

Twigs of plants (leaf buds, tender leaves), algae

≥ 300 mg

Plant

Old leaves, roots, stems, bark of plants

≥ 1 g

Plant

Plant buds, pollen

≥ 100 mg

Plant

Plant seeds (rice/wheat seeds, etc.),
fruits (apples, peaches, pears)

≥ 1 g

Microorganism

Prokaryotic bacteria (E. coli, Staphylococcus aureus, etc.),
fungi (yeast, etc.)

Thallus ≥ 50 mg
cells ≥ 5×10^6

Protein solution

Complex protein solution, protein powder

≥ 100 µg,
≥ 0.5 µg/µL

Others

Feces

≥ 300 mg

Data Analysis

Data Analysis

  • Data analysis and validation performed with Mascot

  • Protein identification and quantification list

  • Differential proteins data statistics and volcano plot

  • Principal component analysis (PCA) and cluster analysis

  • Protein GO/COG/KOG/Pathway annotation

  • GO/COG/KOG enrichment analysis of differential proteins

  • Protein-protein interaction analysis

  • Protein subcellular localization analysis

  • Repeatability analysis

Resources

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

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