DESCRIPTION
SPECIFICATION
RESOURCES
Project Workflow
- Protein extraction
- Protein digestion
- Isobaric labeling
- HPLC fractionation
- LC-MS/MS (DDA)
- Data analysis
How to order
Mass Spectrometry Service Specification
Isobaric Label Quantitative Proteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF/HF-X).
Sample Preparation and Services
- The QC of enzymatic hydrolysis and labeling steps are strictly controlled by LC-MS DDA analysis
- 1h/fraction; 20 fractions for eukaryotes;10 fractions for prokaryotes
Quality Standard
- Summary includes all methods and data analysis
- Reports provided in Excel or PDF format, RAW files available upon request
Turn Around Time
- Typical 4-5 weeks from sample QC acceptance to data report delivery
| SAMPLE TYPE | SAMPLE TYPE | RECOMMEND AMOUNT |
|---|---|---|
| Animal | Common animal tissues: animal internal organs (heart, liver, spleen, lung, kidney), skin, muscle, brain, etc | ≥ 10 mg |
| Animal | Mollusks (Toxoplasma, Schistosomiasis, Drosophila, Acarid, Plutella xylostella, Laodelphax, Cestode, Cicada, Hematodinium, etc.) | ≥ 10 mg |
| Cell | Suspended cells, adherent cells | ≥ 1×10^7 |
| Cell | Cell culture supernatant | ≥ 10 mg |
| Exosome | Exosome isolated by customer | ≥ 50 µg, ≥ 0.5 µg/µL |
| Fluid | Plasma, serum (remove highly-abundant protein) | ≥ 200 µL |
| Fluid | Plasma, serum (with highly-abundant protein) | ≥ 10 µL |
| Fluid | Amniotic fluid, cerebrospinal fluid, semen, etc. (remove highly-abundant protein) | ≥ 1 mL |
| Fluid | Amniotic fluid, cerebrospinal fluid, semen, etc. (with highly-abundant protein) | ≥ 200 µL |
| Fluid | Saliva, milk | ≥ 200 µL |
| Fluid | Urine | ≥ 30 mL |
| Plant | Tear | ≥ 15 µL |
| Plant | Twigs of plants (leaf buds, tender leaves), algae | ≥ 300 mg |
| Plant | Old leaves, roots, stems, bark of plants | ≥ 1 g |
| Plant | Plant buds, pollen | ≥ 100 mg |
| Plant | Plant seeds (rice/wheat seeds, etc.), fruits (apples, peaches, pears) | ≥ 1 g |
| Microorganism | Prokaryotic bacteria (E. coli, Staphylococcus aureus, etc.), fungi (yeast, etc.) | Thallus ≥ 50 mg cells ≥ 5×10^6 |
| Protein solution | Complex protein solution, protein powder | ≥ 100 µg, ≥ 0.5 µg/µL |
| Others | Feces | ≥ 300 mg |
Data Analysis
DATA ANALYSIS
- Data analysis and validation performed with Mascot
- Protein identification and quantification list
- Differential proteins data statistics and volcano plot
- Principal component analysis (PCA) and cluster analysis
- Protein GO/COG/KOG/Pathway annotation
- GO/COG/KOG enrichment analysis of differential proteins
- Protein-protein interaction analysis
- Protein subcellular localization analysis
- Repeatability analysis
Resources
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
