Lipidomics Profiling

Service Description

BGI lipidomics profiling services are designed to obtain a lipid profile and screen for differentially-expressed lipids in the sample. BGI has extensive experience in the field of lipidomics profiling with developed reliable workflows using market leading technologies and a bioinformatics infrastructure that is second to none1.

Lipidomics Profiling

Combined with LipidSearch software and advanced algorithm analysis, BGI’s lipidomics profiling service enables confirmation of lipid structure characteristics such as lipid molecule head structure, fatty acid branch chain length and unsaturated double bond number by comparing the characteristic signals of precursor ions, MS2 fragments and neutral loss. LipidSearch is equipped with the largest lipids database, covering 8 categories of lipids, 300 lipid subclasses, and containing more than 1.7 million lipid ions and their predictive fragment ions information.
 

Project Workflow

References

[1] Zhong H, et al., Lipidomic profiling reveals distinct differences in plasma lipid composition in healthy, prediabetic, and type 2 diabetic individuals. Gigascience, 2017 07 01; 67(7). doi:10.1093/gigascience/gix036.

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Mass Spectrometry Service Specification

Lipidomics Profiling services are performed using Waters ACQUITY UPLC and cutting-edge mass spectrometer (Q Exactive HF/HF-X).

  • Sample Preparation and Services

    Sample Preparation and Services

    • Increase metabolome depth of coverage using ACQUITY UPLC CSH C18 column
    • (+) and (-) ESI detection by a Q Exactive HF/HF-X mass spectrometer
  • Quality Standard

    Quality Standard

    • Summary includes all methods and data analysis
    • Reports provided in Excel or PDF format; RAW files available upon request
  • Turn Around Time

    Turn Around Time

    • Typical 3-5 weeks after sample receipt for raw data delivery

Sample Requirements

We accept lipid samples in a variety of formats. Contact your BGI account representative for details for your samples.

Sample Type

Recommended Sample Amount

Minimum Sample Amount

Serum, plasma

≥ 250 µL

≥ 50 µL

Urine

≥ 500 µL

≥ 50 µL

Animal and clinical tissues

≥ 200 mg

≥ 25 mg

Feces and intestinal contents

≥ 200 mg

≥ 50 mg

Cell

≥ 1×10^7

≥ 5×10^6

Microorganism

≥ 1×10^7 or ≥ 100 mg

≥ 5×10^6 or ≥25 mg

Culture medium,
fermentation medium

≥ 1 mL

≥ 100 µL

Plant tissue

≥ 1 g

≥ 100 mg

Milk

≥ 1 mL

≥ 1 mL

Other body fluids (amniotic fluid, saliva, hemolymph, cerebrospinal fluid, etc.)

≥ 250 µL

≥ 50 µL

Data Analysis

  • Data analysis performed with metaX1 and BGI library

  • T-test, PCA, PLS-DA for differential metabolic identification

  • Cluster analysis

  • Metabolite pathway annotation

  • ROC analysis

  • Metabolite correlation analysis

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