Metabolomics

Metabolomics

Service Description

Metabolomics is the scientific study of chemical processes involving metabolites, the small molecule substrates, intermediates and products of metabolism that occur within cells, biofluids, tissues or organisms. Collectively, these small molecules and their interactions within a biological system are known as the metabolome.

Metabolites and their concentrations directly reflect the underlying biochemical activity and state of cells and tissues. BGI’s metabolomic solutions therefore provide researchers a way to study the ‘chemical fingerprint’ that specific cellular processes leave after they have finished. 

BGI has extensive experience in the field of metabolomics and offers a wide range of services including overall metabolite profiles as well as targeted small molecule analysis services.

By applying state-of-the-art LC-MS/MS systems and techniques, BGI offers validated metabolomics workflows including:

  • Untargeted metabolomics (water/lipid-soluble)
  • Targeted small molecule analysis (vitamins, amino acids, hormones and 700+ kinds of small molecules)

 

Instrumentation

Liquid chromatography system: Waters ACQUITY UPLC I-Class
Liquid chromatography column: Waters C18, HILIC
Orbitrap: Thermo Q Exactive HF, Q Exactive
Triple Quad: SCIEX QTRAP4500/5500/6500, Waters Xevo TQS, TQD
Q-TOF: Waters Xevo G2-XS QTOF
GC-MS: Agilent 7890B GC System/5977A MSD, Thermo TRACE 1300/TSQ 9000
ICP-MS: Agilent 7700 Series

BGI Metabolomics Service instrumentation

 

 

Targeted Small Molecule Analysis Solutions

BGI’ s targeted metabolomics service involves the detection and analysis for specific metabolites. By quantifying the small molecules with reference to established standards, BGI’ s targeted metabolomics service can accurately monitor the dynamic metabolic process, uncover relevant metabolic mechanisms and verify potential metabolic biomarkers.

Project Workflow:

 

 

Untargeted Metabolomics Solutions

BGI provides LC-MS/MS untargeted metabolomics services to support biomedical and biotechnology applications. Our Untargeted Metabolomics services are designed to obtain a metabolite profile and screen for differentially-expressed molecules in the sample.

We provide panels for both standard metabolome profiling (water-soluble metabolites, amino acids, organic acids, amines, etc.) and lipidome profiling (polar and non-polar lipids, lipidic metabolites, etc.)

Project Workflow:

BGI Untargeted Metabolomics Solutions workflow

 

Selected Publications

Nature Communications
Analyses of gut microbiota and plasma bile acids enable stratification of patients for antidiabetic treatment

Nature Medicine
Gut microbiome and serum metabolome alterations in obesity and after weight-loss intervention

GigaScience
Lipidomic profiling reveals distinct di erences in plasma lipid composition in healthy, prediabetic and type 2 diabetic individuals[3]

GigaScience
Lipidomics profiling reveals the role of glycerophospholipid metabolism in psoriasis

Scientific reports
Integrated metabolomics and metagenomics analysis of plasma and urine identified microbial metabolites associated with coronary heart disease

 

Request for Information or Quotation

Contact your BGI account representative for the most affordable rates in the industry and to discuss how we can meet your specific project requirements or for expert advice on experiment design, from sample to bioinformatics.

Metabolomics Data Analysis

After mass spectrometry data acquisition, we use the commercial software Progenesis QI (version 2.2) (Waters, UK) and the self-developed metabolomics R software package metaX[2] to undertake statistical analysis of mass spectral data, wherein metabolite identification is based on the databases KEGG, HMDB and LIPID MAPS.

Metabolomics data analysis

Metabolomics data analysis 1

Metabolomics data analysis 2

Metabolomics data analysis 3

Service Specification

BGI proteomics services are available from our state-of-the-art laboratories in China, where experienced personnel care for your samples.

  • Turn Around Time

    Turn Around Time

    • Typical 25 working days after sample receipt for raw data delivery

Sample Requirements

We can process a large variety of tissue and cell sample types. Contact your BGI account representative for details for your samples.

Service Overview and Case Study

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