Sample Preparation and Services
- Enrichment of endogenous polypeptides
- 1h for LC-MS/MS analysis
We have developed innovative solutions for optimizing the process of comprehensive qualitative and quantitative analysis of all endogenous peptides in complex biological matrices and other complex mixtures.
Peptidomics is an emerging field filling the research gap between proteomics and metabolomics and can be defined as the comprehensive qualitative and quantitative analysis of all endogenous peptides from biological samples at a specific time and location.
BGI has extensive experience in the field of peptidomics spanning disease biomarker study, off target effects of drugs, food processing and fermentation research, the molecular mechanisms of animals and plants, and cell antigen epitopes prediction1,2.
 Chen X Y, et al., High-Throughput Identification of Putative Antimicrobial Peptides from Multi-Omics Data of the Lined Seahorse (Hippocampus erectus). Mar. Drugs, 2020, 18(1), 30. doi: 10.3390/md18010030.
 Yi Y H, et al., High Throughput Identification of Antihypertensive Peptides from Fish Proteome Datasets. Mar Drugs, 2018 Oct 2;16(10):365. doi: 10.3390/md16100365.
Peptidomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF and Orbitrap Fusion Lumos).
We accept samples in a variety of formats.
100 µL/one experiment
2 mL/one experiment
20 mL/one experiment
For complex polypeptide samples, the sample amount of molecular weight less than 10kDa is > 2 μg (refer to A280 detection value), and the purity is > 90%. And it does not contain special and hard-to-remove substances, such as SDS, Triton x, chaps, etc
Data analysis and validation performed with Mascot
Identification of peptides and proteins
Protein GO/COG/Pathway annotation
Identified peptide matching analysis of specific protein