Sample Preparation and Services
- Digestion performed using sequencing-grade trypsin
- 60 min nano-flow LC-MS/MS
Modern liquid chromatography and mass spectrometry technology provides a means to routinely perform non-targeted protein identification1.
BGI can help simplify the challenge of protein identification for your project.
Gel Spot Identification
We can identify proteins present in gel spots.
Gel Band Identification
We can identify proteins present in gel bands or protein solution.
. Baldwin MA. Protein identification by mass spectrometry: issues to be considered. Mol Cell Proteomics. 2004 Jan;3(1):1-9. Epub 2003 Nov 6. Review. PubMed PMID: 14608001.
Projects that need enrichment for acetylation and ubiquitination studies is not accepted.
Coomassive or silver stained protein spot/band
Protein ≥ 1 μg
Protein ≥ 1μg. Projects that need enrichment is not accepted.
Single protein (protein solution purified after IP or CO-IP)
≥ 5 μg,≥ 0.5 μg/μL
≥ 10 μg, ≥ 0.5 μg/μL. If enrichment is required, the amount of sample sent needs to be increased by 3 times.
Data analysis and validation performed with Mascot
GO (Gene ontology) category analysis
COG (Cluster of Orthologous Groups of proteins) category analysis
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.