Protein Identification

Service Description

Modern liquid chromatography and mass spectrometry technology provides a means to routinely perform non-targeted protein identification1.

BGI can help simplify the challenge of protein identification for your project.

Project Workflow

Gel Spot Identification
We can identify proteins present in gel spots.

  • Gel spot
  • Decolorization
  • Protein digestion
  • LC-MS/MS (DDA)
  • Protein identification

Gel Band Identification
We can identify proteins present in gel bands or protein solution.

  • Gel band or protein solution
  • Decolorization
  • Protein digestion
  • LC-MS/MS (DDA)
  • Bioinformatics analysis

References

[1]. Baldwin MA. Protein identification by mass spectrometry: issues to be considered. Mol Cell Proteomics. 2004 Jan;3(1):1-9. Epub 2003 Nov 6. Review. PubMed PMID: 14608001.

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Service Specification

  • Sample Preparation and Services

    Sample Preparation and Services

    • Digestion performed using sequencing-grade trypsin
    • 60 min nano-flow LC-MS/MS
  • Quality Standard

    Quality Standard

    • Summary includes all methods and data analysis
    • Reports provided in Excel or PDF format, RAW files available upon request
  • Turn Around Time

    Turn Around Time

    • Typical 2 weeks after sample receipt for raw data delivery

Sample Requirements

Projects that need enrichment for acetylation and ubiquitination studies is not accepted.

Product Sample

Gel bandidentification

Modification site identification

Coomassive or silver stained protein spot/band

Protein ≥ 1 μg

Protein ≥ 1μg. Projects that need enrichment is not accepted.

Single protein (protein solution purified after IP or CO-IP)

≥ 5 μg,≥ 0.5 μg/μL

≥ 10 μg, ≥ 0.5 μg/μL. If enrichment is required, the amount of sample sent needs to be increased by 3 times.

Data Analysis

ADVANCED BIOINFORMATICS

  • Data analysis and validation performed with Mascot

  • GO (Gene ontology) category analysis

  • COG (Cluster of Orthologous Groups of proteins) category analysis

  • Pathway analysis

Resources

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

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