Protein Identification

Protein Identification

Our protein identification services mainly include Protein Identification (Gel Spot/Gel Band Identification) and Proteome Profiling.

We are happy to work with you to determine the best qualitative proteomics strategy for your project1.

Proteome Profiling

Proteome profiling is a cost effective, high value solution for monitoring hundreds-to-thousands of proteins simultaneously.

We perform sample digestion using sequencing-grade trypsin or alternative proteolytic method, and then trypsin-digested peptide samples are analyzed using nano-flow LC-MS/MS. The spectral counting method provides information on protein relative abundances2. Besides, our HPLC sample fractionation systems are highly recommended for complex sample types to reduce sample complexity and maximize protein sequence coverage and detection dynamic range3.

Reference

[1] Baldwin MA. Protein identification by mass spectrometry: issues to be considered. Mol Cell Proteomics. 2004 Jan;3(1):1-9. Epub 2003 Nov 6. Review. PubMed PMID: 14608001.
[2] Zhang, Y., et al., Tissue-Based Proteogenomics Reveals that Human Testis Endows Plentiful Missing Proteins. Journal of Proteome Research, 2015. 14(9): p. 3583-3594.
[3] Chandramouli, K. and P.Y. Qian, Proteomics: challenges, techniques and possibilities to overcome biological sample complexity. Hum Genomics Proteomics, 2009. 2009

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