Proteome profiling analysis refers to the analysis of an entire proteome from complex samples like complete cells, tissues and body fluids. It is most often used to identify as many peptides and proteins as possible. Proteome profiling analysis based on mass spectrometry (MS) can provide reference information for high-throughput quantitative proteomics and protein modification analysis.
Proteome profiling is a cost effective, high value solution for monitoring hundreds-to-thousands of proteins simultaneously.
We perform sample digestion using sequencing-grade trypsin or alternative proteolytic method, and then trypsin-digested peptide samples are analyzed using nano-flow LC-MS/MS. The spectral counting method provides information on protein relative abundances1. Besides, our HPLC sample fractionation systems are highly recommended for complex sample types to reduce sample complexity and maximize protein sequence coverage and detection dynamic range2.
 Zhang, Y., et al., Tissue-Based Proteogenomics Reveals that Human Testis Endows Plentiful Missing Proteins. Journal of Proteome Research, 2015. 14(9): p. 3583-3594.
 Chandramouli, K. and P.Y. Qian, Proteomics: challenges, techniques and possibilities to overcome biological sample complexity. Hum Genomics Proteomics, 2009. 2009