Rapid DDA Proteomics

Service Description

We have developed label-free data-dependent acquisition (DDA) proteomics solutions for quantifying proteins in matrices with low complexity.


Rapid DDA Proteomics

In contrast to label-dependent quantitative proteomics such as iTRAQ method, label-free DDA proteomics doesn’t need to use expensive a stable isotope for labeling of proteins, which allows accurate protein quantification of samples without additional error-prone in vitro labelling reactions and enables fast proteome identification and quantification1.

Project Workflow

  • Protein extraction
  • Protein digestion
  • HPLC fractionation
  • LC-MS/MS (DDA)
  • Data analysis


[1] Krasny L, et al., Data-independent acquisition mass spectrometry (DIA-MS) for proteomic applications in oncology. Mol Omics, 2020. 9(4).

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

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Mass Spectrometry Service Specification

Rapid DDA Proteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF/HF-X and Triple TOF 5600).

  • Sample Preparation and Services

    Sample Preparation and Services

    • The QC of enzymatic hydrolysis is strictly controlled by LC-MS
    • 2h for DDA analysis
  • Quality Standard

    Quality Standard

    • Summary includes all methods and data analysis
    • Reports provided in Excel or PDF format, RAW files available upon request
  • Turn Around Time

    Turn Around Time

    • Typical 3-4 weeks from sample QC acceptance to data report delivery

Sample type

Sample type

Recommend Amount


Common animal tissues:
animal internal organs (heart, liver, spleen, lung, kidney), skin, muscle, brain, etc

≥ 5 mg


(Toxoplasma, Schistosomiasis, Drosophila, Acarid, Plutella xylostella, Laodelphax, Cestode, Cicada, Hematodinium, etc.)

≥ 5 mg


Suspended cells, adherent cells

≥ 1×10^5


Cell culture supernatant

≥ 5 mL


Exosome isolated by customer

≥ 10 µg, ≥ 0.5 µg/µL


Plasma, serum (remove highly-abundant protein)

≥ 200 µL


Plasma, serum (with highly-abundant protein)



Amniotic fluid, cerebrospinal fluid, semen, etc. (remove highly-abundant protein)

≥ 1 mL


Amniotic fluid, cerebrospinal fluid, semen, etc. (with highly-abundant protein)

≥ 100 µL


Saliva, milk

≥ 50 µL



≥ 20 mL



≥ 5 µL


Twigs of plants (leaf buds, tender leaves), algae

≥ 200 mg


Old leaves, roots, stems, bark of plants

≥ 500 mg


Plant buds, pollen

≥ 20 mg


Plant seeds (rice/wheat seeds, etc.),
fruits (apples, peaches, pears)

≥ 500 mg


Prokaryotic bacteria (E. coli, Staphylococcus aureus, etc.),
fungi (yeast, etc.)

Thallus ≥ 50 mg
cells ≥ 5×10^6

Protein solution

Complex protein solution, protein powder

≥ 20 µg,
≥ 0.5 µg/µL



≥ 200 mg

Data Analysis

Data Analysis

  • Data analysis and validation performed with Mascot

  • Protein identification and quantification list

  • Differential proteins data statistics and volcano plot

  • Principal component analysis (PCA) and cluster analysis

  • Protein GO/COG/KOG/Pathway annotation

  • GO/COG/KOG enrichment analysis of differential proteins

  • Protein-protein interaction analysis

  • Protein subcellular localization analysis

  • Time series analysis

  • Repeatability analysis


We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

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