Rapid DDA Proteomics

Service Description

We have developed label-free data-dependent acquisition (DDA) proteomics solutions for quantifying proteins in matrices with low complexity.

 

Rapid DDA Proteomics

In contrast to label-dependent quantitative proteomics such as iTRAQ method, label-free DDA proteomics doesn’t need to use expensive a stable isotope for labeling of proteins, which allows accurate protein quantification of samples without additional error-prone in vitro labelling reactions and enables fast proteome identification and quantification1.

Project Workflow

  • Protein extraction
  • Protein digestion
  • HPLC fractionation
  • LC-MS/MS (DDA)
  • Data analysis

References

[1] Krasny L, et al., Data-independent acquisition mass spectrometry (DIA-MS) for proteomic applications in oncology. Mol Omics, 2020. 9(4).

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

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Mass Spectrometry Service Specification

Rapid DDA Proteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF/HF-X and Triple TOF 5600).

  • Sample Preparation and Services

    Sample Preparation and Services

    • The QC of enzymatic hydrolysis is strictly controlled by LC-MS
    • 2h for DDA analysis
  • Quality Standard

    Quality Standard

    • Summary includes all methods and data analysis
    • Reports provided in Excel or PDF format, RAW files available upon request
  • Turn Around Time

    Turn Around Time

    • Typical 3-4 weeks from sample QC acceptance to data report delivery

Sample type

Sample type

Recommend Amount

Animal

Common animal tissues:
animal internal organs (heart, liver, spleen, lung, kidney), skin, muscle, brain, etc

≥ 5 mg

Animal

Mollusks
(Toxoplasma, Schistosomiasis, Drosophila, Acarid, Plutella xylostella, Laodelphax, Cestode, Cicada, Hematodinium, etc.)

≥ 5 mg

Cell

Suspended cells, adherent cells

≥ 1×10^5

Cell

Cell culture supernatant

≥ 5 mL

Exosome

Exosome isolated by customer

≥ 10 µg, ≥ 0.5 µg/µL

Fluid

Plasma, serum (remove highly-abundant protein)

≥ 200 µL

Fluid

Plasma, serum (with highly-abundant protein)

/

Fluid

Amniotic fluid, cerebrospinal fluid, semen, etc. (remove highly-abundant protein)

≥ 1 mL

Fluid

Amniotic fluid, cerebrospinal fluid, semen, etc. (with highly-abundant protein)

≥ 100 µL

Fluid

Saliva, milk

≥ 50 µL

Fluid

Urine

≥ 20 mL

Fluid

Tear

≥ 5 µL

Plant

Twigs of plants (leaf buds, tender leaves), algae

≥ 200 mg

Plant

Old leaves, roots, stems, bark of plants

≥ 500 mg

Plant

Plant buds, pollen

≥ 20 mg

Plant

Plant seeds (rice/wheat seeds, etc.),
fruits (apples, peaches, pears)

≥ 500 mg

Microorganism

Prokaryotic bacteria (E. coli, Staphylococcus aureus, etc.),
fungi (yeast, etc.)

Thallus ≥ 50 mg
cells ≥ 5×10^6

Protein solution

Complex protein solution, protein powder

≥ 20 µg,
≥ 0.5 µg/µL

Others

Feces

≥ 200 mg

Data Analysis

Data Analysis

  • Data analysis and validation performed with Mascot

  • Protein identification and quantification list

  • Differential proteins data statistics and volcano plot

  • Principal component analysis (PCA) and cluster analysis

  • Protein GO/COG/KOG/Pathway annotation

  • GO/COG/KOG enrichment analysis of differential proteins

  • Protein-protein interaction analysis

  • Protein subcellular localization analysis

  • Time series analysis

  • Repeatability analysis

Resources

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

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