Untargeted Metabolomics

Service Description

BGI has extensive experience in the field of untargeted metabolomics with developed reliable workflows using market leading technologies and a bioinformatics infrastructure that is second to none.

Untargeted Metabolomics

By applying state-of-the-art LC-MS/MS systems and techniques, BGI offers validated workflows of untargeted metabolomics to support biomedical and biotechnology applications1. Our Untargeted Metabolomics services are designed to obtain a metabolite profile and screen for differentially-expressed molecules in the sample.

 

Project Workflow

References

[1] Liu R., et al., Gut microbiome and serum metabolome alterations in obesity and after weight-loss intervention. Nat Med, 2017. 23(7): p. 859-868.

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Mass Spectrometry Service Specification

Untargeted Metabolomics services are performed using Waters ACQUITY UPLC and cutting-edge mass spectrometer (Q Exactive HF/HF-X and Q-TOF 5600).

  • Sample Preparation and Services

    Sample Preparation and Services

    • Increase metabolome depth of coverage using both ACQUITY UPLC BEH C18 and Amide column
    • (+) and (-) ESI detection by a Q Exactive HF/HF-X mass spectrometer
  • Quality Standard

    Quality Standard

    • Summary includes all methods and data analysis
    • Reports provided in Excel or PDF format, RAW files available upon request
  • Turn Around Time

    Turn Around Time

    • Typical 3-5 weeks after sample receipt for raw data delivery

Sample Requirements

We accept metabolite samples in a variety of formats. Contact your BGI account representative for details for your samples.

Sample type

Recommended Sample Amount

Minimum Amount

Serum, plasma

≥ 250 µL

≥ 50 µL

Urine

≥ 500 µL

≥ 50 µL

Animal and clinical tissues

≥ 200 mg

≥ 25 mg

Feces and intestinal contents

≥ 200 mg

≥ 50 mg

Cell

≥ 1×10^7

≥ 5×10^6

Microorganism

≥ 1×10^7 or ≥ 100 mg

≥ 5×10^6 or ≥25 mg

Culture medium,
fermentation medium

≥ 1 mL

≥ 100 µL

Plant tissue

≥ 1 g

≥ 100 mg

Milk

≥ 1 mL

≥ 1 mL

Other body fluids (amniotic fluid, saliva, hemolymph, cerebrospinal fluid, etc.)

≥ 250 µL

≥ 50 µL

Data Analysis

  • Data analysis performed with metaX1 and BGI library

  • T-test, PCA, PLS-DA for differential metabolite identification

  • Cluster analysis

  • Metabolite pathway annotation

  • ROC analysis

  • Metabolite correlation analysis

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