10X Genomics Single Cell Sequencing

10X Genomics Single Cell Sequencing

10X Genomics Single Cell Sequencing Service Description

Cell populations are heterogeneous and unsynchronized in their characteristics. Traditional bulk RNA sequencing generates comprehensive information but does not provide insight into variable expression between cells. 10X Genomics Chromium Single Cell 3′ RNA-seq provides a scalable solution for cell characterization and gene expression profiling of hundreds to millions of cells. 10X Genomics technology is able to handle a large quantity of single cells fast and accurately, providing research solutions across a wide variety of applications including cancer, neurology, and immunology.

 

DNBseq™ NGS technology platform

BGI’s Single Cell Sequencing service is typically executed with our proprietary DNBseq™ NGS technology platform, for great sequencing data at the lowest cost in the industry.

DNBseq™ is BGI’s proprietary sequencing technology, developed by our Complete Genomics subsidiary in Silicon Valley. This system is powered by combinatorial Probe-Anchor Synthesis (cPAS), linear isothermal Rolling-Circle Replication and DNA Nanoballs (DNBTM) technology, followed by high-resolution digital imaging.

DNBseq BGI sequencing technology

The major applications of single cell sequencing include:

Project Workflow

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

  • Sample QC
  • Library QC
  • Sequencing QC
  • Data QC
  • Single Cell Preparation
  • Library Preparation
  • Library Circularisation
  • Sequencing
  • Bioinformatics

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Sequencing Service Specification

BGI's 10X Genomics Single Cell Sequencing services are performed on our DNBseq technology platform:

  • Sequencing

    Sequencing

    • Guaranteed ≥90% of bases with quality score of ≥Q20
    • DNBseq PE100 or Illumina PE150 options
    • Raw data and bioinformatics analysis are available in standard file formats
  • Turnaround Time

    Turnaround Time

    • Typical 35 working days from sample QC acceptance to filtered raw data availability
    • Expedited services are available, contact your local BGI specialist for details

Sample Requirements

  • Cell line/Tissue/PBMC:

    Cell line/Tissue/PBMC:

    • After sample processing, freeze cells at a final concentration of 1x106 cells/ml in cryopreserved tubes (Cell freezing medium: 90% FBS+10% DMSO)

Data Analysis

In addition to raw data output, BGI offers a range of standard and customized bioinformatics pipelines for your single cell sequencing project.

Standard Analysis for 10X Genomics Single Cell 3’ RNA-seq

  • Reads extraction: Extract cell-barcodes and UMIs from reads

  • Reads alignment: Align short reads to reference genome and transcriptome

  • UMI counting: Form a raw-gene-barcode matrix according to UMI number

  • Barcoding filtering: Filter those barcodes not from cellular GEM

  • Matrix formation: Form filtered gene-barcode matrix

  • PCA de-dimension: Main component analysis (keep most highly variable components)

  • Clustering: Graph-based clustering on PCA analysis

  • Clustering: Kmeans clustering on PCA analysis

  • T-SNE de-dimension: Visualize PAC result in 2D dimension by t-SNE de-dimension

  • DEG analysis: Identify the most significant differential expression gene in each cluster

  • Generate report: Generate a report in CSV and HTML format

Customized analysis

Further customization of bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative

Single Cell DNA/RNA Sequencing Resources

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