Single cell sequencing is used for solving problems associated with the study of heterogeneous samples, rare cell types, cell lineage relationships, and disease evolution. By using deep sequencing of DNA and RNA from single cell, cellular functions of individual cells can be investigated. BGI applies the superior method of Multiple Displacement Amplification (MDA) for single cell genomic DNA amplification (WGS/WES), and Switching Mechanism at 5′ End of RNA Template (SMART-seq2) for single cell RNA amplification (transcriptome/RNA quantification).
DNBSEQ™ NGS technology platform
BGI’s Single Cell Sequencing service is typically executed with our proprietary DNBSEQ™ NGS technology platform, for great sequencing data at the lowest cost in the industry.
DNBSEQ™ is BGI’s proprietary sequencing technology, developed by our Complete Genomics subsidiary in Silicon Valley. This system is powered by combinatorial Probe-Anchor Synthesis (cPAS), linear isothermal Rolling-Circle Replication and DNA Nanoballs (DNBTM) technology, followed by high-resolution digital imaging.
The major applications of single cell sequencing include:
profiling circulating tumor cells
pre-implantation genomic screening of embryos
searching for disease marker
studying tissue and cell group heterogeneity
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.