Whole Genome Bisulfite Sequencing (WGBS) provides a scalable solution for cell characterization and gene expression profiling of hundreds to millions of cells. Tell us about your project, our experts are here to help.
Methylation of DNA at the fifth position in cytosine (5-mC) is a stable epigenetic modification and plays an important role in many biological processes, including gene silencing, suppression of transposable elements, genomic imprinting and X chromosome inactivation. Detection and quantification of methylation are critical to understand gene expression and other processes subjected to epigenetic regulation.
Whole genome bisulfite sequencing (WGBS) is used to detect methylated cytosines by treating the DNA with sodium bisulfite before sequencing. WGBS has become the gold standard for studying genome-wide methylation at single base resolution.
Sample Requirements
Recommended
- Intact genomic DNA: ≥ 1μg
- Concentration: ≥ 50 ng/μl
- Volume: 15μl
Required
- Intact genomic DNA: ≥ 100ng
- Concentration: ≥ 7 ng/μl
- Volume: 15μl
Full sample guidance is provided within the BGI Customer Account Portal.
Sequencing Standards
•100bp paired end sequencing
•Library preparation, including bisulfite treatment
•Guaranteed ≥90% of clean bases with quality score of Q20
•Guaranteed ≥ 99% bisulfite conversion rate
Key Service Details
• Typical 40 working days from sample QC acceptance to filtered raw data availability
• Expedited services are available, contact your local BGI specialist for details
• Reports and output data files are delivered in industry standard file formats: BAM, .xls, .png and FASTQ data.
BGI's Whole Genome Bisulfite Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ is a proprietary sequencing technology, first developed by BGI's Complete Genomics subsidiary in Silicon Valley and offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the index hopping rate in DNBSEQ platforms to be much lower when compared to that of other platforms.
Contact us with any general enquiries or questions for our sequencing experts, we are here to help.
Access Further Sequencing Resources
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results. Access more sequencing resources below or sign up for a BGI Customer Account for full access to our range of educational resources and sample guides.
Opening a BGI Customer Account enables you to request a quote and order services from us. It also provides access to our range of educational resources including educational webinars providing practical sequencing tips and our complete sample preparation guides.