Whole Genome Bisulfite Sequencing

Whole Genome Bisulfite Sequencing

Service Description

Methylation of DNA at the fifth position in cytosine (5-mC) is a stable epigenetic modification and plays an important role in many biological processes, including gene silencing, suppression of transposable elements, genomic imprinting and X chromosome inactivation. Detection and quantification of methylation are critical to understand gene expression and other processes subjected to epigenetic regulation.

Whole genome bisulfite sequencing (WGBS) is used to detect methylated cytosines by treating the DNA with sodium bisulfite before sequencing. WGBS has become the gold standard for studying genome-wide methylation at single base resolution.

  • Cancer
    • Breast cancer
    • Bladder cancer
    • Lymph cancer
    • Liver cancer,etc.
  • Complex diseases
    • Autoimmune disease
    • Cardiovascular disease
    • Mental disorder
    • Genetic imprinting disease, etc.
  • Others
    • Cell differentiation
    • Embryonic development
    • Age and longevity
    • Methylation map, etc.


Choice of Platforms for WGBS

BGI Whole Genome Bisulfite Sequencing services are executed with the DNBSEQ™ sequencing technology, or exclusively with the Illumina sequencing systems, for great sequencing data at the resonable cost in the industry.

DNBSEQ™ is BGI’s proprietary sequencing technology, developed by our Complete Genomics subsidiary in Silicon Valley.

Give our DNBSEQ WGBS services a try and find out why so many trust their valuable research samples to the world-class sequencing experience of BGI.

Ask your BGI representative about our current introductory pricing for WGBS with the BGI’s own BGISEQ sequencing technology.

Project Workflow

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

  • Sample QC
  • Library QC
  • Sequencing QC
  • Data QC
  • Sample preparation
  • Library construction
  • Sequencing
  • Raw data output
  • Bioinformatics analysis

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Sequencing Service Specification

Whole Genome Bisulfite Sequencing services are performed using the DNBSEQ platform. HiSeq services are available when required.

  • Sample Preparation and Services

    Sample Preparation and Services

    • 100bp paired end sequencing
    • Library preparation, including bisulfite treatment
  • Sequencing Quality Standard

    Sequencing Quality Standard

    • Guaranteed ≥90% of clean bases with quality score of Q20
    • Guaranteed ≥ 99% bisulfite conversion rate
  • Turn Around Time

    Turn Around Time

    • Typical 40 working days from sample QC acceptance to filtered raw data availability
    • Expedited services are available, contact your local BGI specialist for details

Sample Requirements

We can process your gDNA samples, whole blood, cell line, fresh frozen tissue and FFPE samples and single-cell applications, with the following general requirements:

Purity: OD260/280 ≥ 1.8 ~ 2.0 without degradation or RNA contamination

DNA Amount and Concentration

Minimum Sample Volume

Regular Samples

Intact genomic DNA ≥ 1 µg, Concentration ≥ 50 ng/µl


Low Input Samples

Intact genomic DNA ≥ 100 ng, Concentration ≥ 7 ng/µl


Data Analysis

Standard Analysis

  • Data Filtering

  • Alignment

  • Data Quality Statistics

  • Methyl-cytosine identification

  • Differential Methylated Region (DMR) analysis

  • Statistics of methylation level


BGI offers extensive analysis options providing unmatched flexibility in delivering reliable data for achieving various research aims ranging from cancer research to understanding basic cellular processes.

WGBS Resources

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

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