Sample Preparation and Services
- 100bp paired end sequencing
- Library preparation, including bisulfite treatment
Methylation of DNA at the fifth position in cytosine (5-mC) is a stable epigenetic modification and plays an important role in many biological processes, including gene silencing, suppression of transposable elements, genomic imprinting and X chromosome inactivation. Detection and quantification of methylation are critical to understand gene expression and other processes subjected to epigenetic regulation.
Whole genome bisulfite sequencing (WGBS) is used to detect methylated cytosines by treating the DNA with sodium bisulfite before sequencing. WGBS has become the gold standard for studying genome-wide methylation at single base resolution.
BGI Whole Genome Bisulfite Sequencing services are executed with the DNBSEQ™ sequencing technology, or exclusively with the Illumina sequencing systems, for great sequencing data at the resonable cost in the industry.
DNBSEQ™ is BGI’s proprietary sequencing technology, developed by our Complete Genomics subsidiary in Silicon Valley.
Give our DNBSEQ WGBS services a try and find out why so many trust their valuable research samples to the world-class sequencing experience of BGI.
Ask your BGI representative about our current introductory pricing for WGBS with the BGI’s own BGISEQ sequencing technology.
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.
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Whole Genome Bisulfite Sequencing services are performed using the DNBSEQ platform. HiSeq services are available when required.
We can process your gDNA samples, whole blood, cell line, fresh frozen tissue and FFPE samples and single-cell applications, with the following general requirements:
Purity: OD260/280 ≥ 1.8 ~ 2.0 without degradation or RNA contamination
Regular Samples
Intact genomic DNA ≥ 1 µg, Concentration ≥ 50 ng/µl
15μl
Low Input Samples
Intact genomic DNA ≥ 100 ng, Concentration ≥ 7 ng/µl
15μl
Data Filtering
Alignment
Data Quality Statistics
Methyl-cytosine identification
Differential Methylated Region (DMR) analysis
Statistics of methylation level
BGI offers extensive analysis options providing unmatched flexibility in delivering reliable data for achieving various research aims ranging from cancer research to understanding basic cellular processes.
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
