BGI is a recognized leader in De Novo Genome Sequencing and has extensive experience from the de novo sequencing and assembly of more than 400 species’ genomes. Contact us today with any questions or for a no obligation quote.
De novo sequencing refers to the sequencing of a novel genome without a reference sequence for alignment. The process of de novo genome sequencing involves the sequencing of DNA fragments, assembling the reads into longer sequences (contigs) and finally ordering the contigs to obtain the entire genome sequence.
BGI has been providing de novo whole genome sequencing for over 20 years and are responsible for the initial publication of countless de novo genomes across a huge variety of species in the world's leading journals.
We offer a complete suite of technologies to support your de novo sequencing projects, along with expert assistance with the planning of optimal sequencing and bioinformatics options, to assure your project is a success.
Sample Requirements
Various libraries offered for DNBSEQ, Illumina, Nanopore PromethION, PacBio Sequel II sequencing.
Most Common Libraries:
DNBSEQ 350bp library:
- Mass≥1μg
- Concentration 20 ng/ul
DNBSEQ stLFR library:
- Mass≥500 ng
- Concentration1 ng/ul
Full sample guidance is provided within the BGI Customer Portal.
Sequencing Standards
• Guaranteed >90% of DNBSEQ™ clean bases with quality score of Q20
• Guaranteed >50Gb Nanopore pass data with Q>7
• Guaranteed >100Gb PacBio Sequel II CLR data
• Guaranteed >20Gb PacBio Sequel II CCS (HiFi library) data with accuracy greater than 99%, except some complex species
Key Service Details
For the species with genome size ≤ 5Gb:
- 70 working days from sample QC acceptance to filtered data available；
- 40/70 working days for the bioinformatics of common/complex genome assembly；
- 30 working days for the bioinformatics of genome annotation；
Case by case for species with genome size > 5Gb.
Reports and output data files are delivered in industry standard file formats: BAM, .xls, .png and FASTQ data.
BGI provides various combinations of sequencing platforms, sequencing read-lengths and paired-end library options for De Novo sequencing applications for plant and animal. Services are performed on DNBSEQ™, Illumina, Nanopore PromethION, PacBio Sequel II or Sequel platforms.
Besides clean data output, BGI offers a range of standard and customized bioinformatics pipelines for your plant and animal de novo sequencing project covering: GENOME SURVEY, GENE ANNOTATION, EVOLUTION ANALYSIS and AUXILIARY ASSEMBLY.
Reports and output data files are delivered in industry standard file formats: BAM, .xls, .png. Raw FASTQ and FASTA data is available.
Contact us with any general enquiries or questions for our sequencing experts, we are here to help.
Access Further Sequencing Resources
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results. Access more sequencing resources below or sign up for a BGI Customer Account for full access to our range of educational resources and sample guides.
Opening a BGI Customer Account enables you to request a quote and order services from us. It also provides access to our range of educational resources including educational webinars providing practical sequencing tips and our complete sample preparation guides.