Sample Preparation and Services
- Library preparation (DNBSEQ™, Illumina, Nanopore PromethION, PacBio Sequel II)
- Various sequencing mode
- Raw data, standard and customized data analysis
- Available data storage and bioinformatics application
De novo sequencing refers to the sequencing of a novel genome without a reference sequence for alignment. The process of de novo genome sequencing involves the sequencing of DNA fragments, assembling the reads into longer sequences (contigs) and ﬁnally ordering the contigs to obtain the entire genome sequence.
BGI is a recognized leader in de novo Whole Genome Sequencing and has extensive experience from the de novo sequencing and published more than 400 species’ genomes. Currently, more than 1000 plant and animal genomes have been sequenced by BGI and its partners (including unpublished species)
We offer a complete suite of technologies to support your de novo sequencing projects, along with expert assistance with the planning of optimal sequencing and bioinformatics options, to assure your project is a success.
We care for your samples from receipt through to result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
BGI provides various combinations of sequencing platforms, sequencing read-lengths and paired-end library options for De Novo sequencing applications for plant, animal and microorganisms.For microorganisms, BGI only provides library construction and sequencing for De Novo whole genome sequencing applications.
Services are performed on DNBSEQ™, Illumina, Nanopore PromethION, PacBio Sequel II or Sequel platforms.
Note: The total quantity of sample required is also determined by the experimental strategy, as well as the type and number of libraries to be constructed.
|PLATFORM||SAMPLE TYPE||MASS||CONCENTRATION||OD||INTEGRITY (AGE)|
|DNBSEQ||stLFR library||≥500 ng||≥1ng/μL||OD260/280: 1.6-2.2 OD260/230: 1.6-2.2||No degradation or little degradation with main band≥40Kb|
|350bp library||≥1μg||≥12.5ng/μL||–||No degradation or little degradation with main band≥20Kb|
|Nanopore PromethION||20-40Kb library||≥9μg||≥90ng/μL||OD260/280: 1.6-2.2 OD260/230: 1.6-2.2||No degradation or little degradation with main band≥40Kb|
|Ultra-long library(>40K)||≥10μg||≥100ng/μL||No degradation or little degradation with main band≥50Kb|
|PacBio Sequel II||15-20Kb HIFI library||≥15μg||≥80ng/μL||OD260/280: 1.6-2.2 OD260/230: 1.6-2.5||No degradation or little degradation with main band≥30Kb|
|20-40Kb CLR library||≥7μg||≥80ng/μL||No degradation or little degradation with main band≥40Kb|
|40-60Kb CLR library||≥9μg||≥80ng/μL||No degradation or little degradation with main band≥50Kb|
Besides clean data output, BGI offers a range of standard and customized bioinformatics pipelines for your plant and animal de novo sequencing project.
Reports and output data flies are delivered in industry standard file formats: BAM, .xls, .png. Raw FASTQ and FASTA data is available.
External pollution Analysis;
Assessment by short reads alignment;
Assembly result correction using long reads;
Assembly result correction using short reads;
Hi-C data auxiliary assembly;
Gene function Annotation;
Gene family identification (≤10 species)；
Phylogenetic tree construction;
Estimation of divergence time;
Genome synteny analysis;
Whole genome duplication analysis;
Gene family expansion and contraction analysis;
Transcription factor prediction;
Specific gene family analysis;
Genome assembly using stLFR data