Sample Preparation and Services
- Library preparation (DNBSEQ™, Illumina, Nanopore PromethION, PacBio Sequel II)
- Various sequencing mode
- Raw data, standard and customized data analysis
- Available data storage and bioinformatics application
De novo sequencing refers to the sequencing of a novel genome without a reference sequence for alignment. The process of de novo genome sequencing involves the sequencing of DNA fragments, assembling the reads into longer sequences (contigs) and finally ordering the contigs to obtain the entire genome sequence.
BGI is a recognized leader in de novo Whole Genome Sequencing and has extensive experience from the de novo sequencing and assembly of more than 400 species’ genomes. Currently, over 70% of higher plant and animal genomes have been sequenced by BGI and its partners (including unpublished species).
We offer a complete suite of technologies to support your de novo sequencing projects, along with expert assistance with the planning of optimal sequencing and bioinformatics options, to assure your project is a success.
We care for your samples from receipt through to result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.
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BGI provides various combinations of sequencing platforms, sequencing read-lengths and paired-end library options for De Novo sequencing applications for plant, animal and microorganisms.For microorganisms, BGI only provides library construction and sequencing for De Novo whole genome sequencing applications.
Services are performed on DNBSEQ™, Illumina, Nanopore PromethION, PacBio Sequel II or Sequel platforms.
Note: The total quantity of sample required is also determined by the experimental strategy, as well as the type and number of libraries to be constructed.
DNBSEQ 350bp library
≥1μg
20 ng/ul
DNBSEQ stLFR library
≥500 ng
1 ng/ul
Nanopore PromethION 20Kb library
≥10μg
100 ng/ul
PacBio Sequel II 20Kb CLR library
≥7μg
60 ng/ul
PacBio Sequel II 15Kb HIFI library
≥15μg
60 ng/ul
PacBio Sequel 20Kb de novo library
≥10μg
60 ng/ul
DNBSEQ 350bp library
-
The band shown on gel electrophoresis has little degradation, or of fragment size greater than 20kb.
DNBSEQ stLFR library
OD260/280: 1.6-2.2 OD260/230: 1.6-2.2
The band shown on gel electrophoresis has little degradation, or of fragment size greater than 40kb.
Nanopore PromethION 20Kb library
OD260/280: 1.8-2.2 OD260/230: 1.8-2.2
The band shown on gel electrophoresis has little degradation, or of fragment size greater than 40kb.
PacBio Sequel II 20Kb CLR library
OD260/280: 1.6-2.2 OD260/230: 1.6-2.2
The band shown on gel electrophoresis has little degradation, or of fragment size greater than 40kb.
PacBio Sequel II 15Kb HIFI library
OD260/280: 1.6-2.2 OD260/230: 1.6-2.2
The band shown on gel electrophoresis has little degradation, or of fragment size greater than 40kb.
PacBio Sequel 20Kb de novo library
OD260/280: 1.6-2.2 OD260/230: 1.6-2.2
The band shown on gel electrophoresis has little degradation, or of fragment size greater than 40kb.
Besides clean data output, BGI offers a range of standard and customized bioinformatics pipelines for your plant and animal de novo sequencing project.
Reports and output data flies are delivered in industry standard file formats: BAM, .xls, .png. Raw FASTQ and FASTA data is available.
Kmer estimation (Jellyfish + GenomeScope)
External pollution Analysis (BWA)
Reads correction
Assembly
Assembly result correction using long reads
Assembly result correction using short reads
BUSCO assessment
Repeat annotation
Gene prediction
Gene function Annotation
Gene family identification (Animal TreeFam; Plant OrthoMCL; ≤10 species);
Phylogenetic tree construction
Estimation of divergence time
Genome synteny analysis
Whole genome duplication analysis
Gene family expansion and contraction analysis
Positive selection analysis
Hi-C data auxiliary assembly
Assembly using stLFR data
Gap filling
Super-Scaffold construction