Over 99% of natural microorganisms can not be isolated and cultured clonally, while traditional isolation and culture-dependent methods challenge the study of microorganisms in their natural environment.
Metagenomic studies of genetic material that is directly recovered from environmental samples, have bene tted greatly from advanced NGS technology as a method for the exploration of microbial biodiversity.
16S and 18S rDNA are hypervariable regions in the 16S or 18S rRNA genes in bacteria and fungus, while ITS (Internal Transcribed Spacer) is the spacer DNA between the small-subunit and large-subunit rRNA genes in bacteria, fungi and archaea.
Sequence comparison of 16S/18S/ITS regions is widely used in taxonomy and molecular phylogeny because of the easy ampli cation by PCR, even from low quantities of DNA, while they have a high degree of variation even between closely related species.
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
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BGI 16S/18S/ITS Amplicon Sequencing services are executed with the Illumina HiSeq 2500 or MiSeq sequencing system.
We can process intact genomic DNA or PCR fragment samples
Genomic DNA
≥50ng
≥ 6ng/μL
PCR Fragment
≥1μg
≥10 ng/μL
In addition to raw data output, BGI offers a range of standard and customized bioinformatics pipelines for your whole genome sequencing project.
Filtering
Overlap paired-end reads to form tags
ags are clustered into OUT. PCA, Venn diagram. A rank curve will be generated based on OTU abundance
Species are classified by OTU annotation, based on which a species profiling histogram, heat map and a phylogeny tree will be provided
Alpha diversity indexes are generated for in single sample
Beta diversity and clustering analysis are performed for multiple samples
Comparative analysis is used to screen significant differences in multiple samples
Further customization of Bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative
