16S/18S/ITS Sequencing

16S/18S/ITS Sequencing

Service Description

Over 99% of natural microorganisms can not be isolated and cultured clonally, while traditional isolation and culture-dependent methods challenge the study of microorganisms in their natural environment.
Metagenomic studies of genetic material that is directly recovered from environmental samples, have bene tted greatly from advanced NGS technology as a method for the exploration of microbial biodiversity.

16S and 18S rDNA are hypervariable regions in the 16S or 18S rRNA genes in bacteria and fungus, while ITS (Internal Transcribed Spacer) is the spacer DNA between the small-subunit and large-subunit rRNA genes in bacteria, fungi and archaea.

Sequence comparison of 16S/18S/ITS regions is widely used in taxonomy and molecular phylogeny because of the easy ampli cation by PCR, even from low quantities of DNA, while they have a high degree of variation even between closely related species.

Project Workflow

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

  • Sample QC
  • Library QC
  • Sequencing QC
  • Data QC
  • Sample Preparation
  • Library Preparation
  • Sequencing
  • Raw Data Output
  • Bioinformatics Analysis

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

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Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Sequencing Service Specification

BGI 16S/18S/ITS Amplicon Sequencing services are executed with the Illumina HiSeq 2500 or MiSeq sequencing system.

  • Sample Preparation and Services

    Sample Preparation and Services

    • PCR will be used to isolate different 16S/18S/ITS regions
    • PE250 sequencing by either MiSeq or HiSeq 2500, and PE300 by MiSeq
    • Clean sequencing data are available in standard file formats
    • Custom bioinformatic data analysis is available
    • Cloud-based data storage and delivery system
  • Sequencing Quality Standard

    Sequencing Quality Standard

    • Up to ≥ 80% of bases with a ≥Q30 quality score, depending on the chosen sequencing strategy
    • Recommended sequencing coverage is dependent on the complexity of the sample
  • Turn Around Time

    Turn Around Time

    • Typical 30 working days from sample QC acceptance to cleaned raw data availability
    • Expedited services are available, contact your local BGI specialist for details

Sample Requirements

We can process intact genomic DNA or PCR fragment samples

DNA concentration

Minimum sample volume

Genomic DNA


≥ 6ng/μL

PCR Fragment


≥10 ng/μL

Data Analysis

In addition to raw data output, BGI offers a range of standard and customized bioinformatics pipelines for your whole genome sequencing project.

Standard Analysis

  • Filtering

  • Overlap paired-end reads to form tags

  • ags are clustered into OUT. PCA, Venn diagram. A rank curve will be generated based on OTU abundance

  • Species are classified by OTU annotation, based on which a species profiling histogram, heat map and a phylogeny tree will be provided

  • Alpha diversity indexes are generated for in single sample

  • Beta diversity and clustering analysis are performed for multiple samples

  • Comparative analysis is used to screen significant differences in multiple samples

Customized analysis

Further customization of Bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative

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