Sample Preparation and Services
- paired-end 100 bp
- Strand specific library with rRNA removal
- Data and bioinformatics analysis are available in standard file formats
- Advanced RNA data visualization and data mining with Dr.Tom system
Long non-coding RNAs (lncRNAs) are a large class of transcribed RNA molecules with a length of more than 200 nucleotides that do not encode proteins. lncRNAs are thought to encompass nearly 30,000 different transcripts in humans, hence lncRNA transcripts account for the major part of the non-coding transcriptome.
lncRNA discovery is still at a an early stage and only a small proportion of lncRNAs have so far been investigated. Although we can start to classify different types of lncRNA functions, we are still far from being able to predict the function of new lncRNAs.
Sequencing Service Description
BGI offers expression profiling as one way to uncover the function of lncRNA. Identifying lncRNAs that are differentially expressed during development or in particular situations can shed light on their potential functions. Alternatively, looking for lncRNAs and protein-coding genes whose expression is correlated, can indicate co-regulation or related functions.
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
DNBSEQ™ Long non-coding RNA Sequencing services are executed with the DNBSEQ™ Technology Platform, with the benefit of lower cost and faster turnaround time.
We can process your total RNA, blood, cell line, FFPE, fresh frozen tissues samples from a variety of species, with the following general requirements.
Purity: OD260/280 ≥ 2.0, OD260/230 ≥ 2.0, without degradation and DNA contamination
Human，mouse and rat
Total RNA ≥200ng,
In addition to data output, BGI offers a range of standard and customized bioinformatics pipelines for your Long non-coding RNA sequencing project. Reports and output data files are delivered in industry standard file formats: FASTQ, BAM and Excel.
Identiﬁcation of mRNA and LncRNA
Quantification and differential expression analysis
Gene ontology analysis and pathway enrichment analysis
Transcription factor prediction (For Animal TFDB/Plant TFDB)
Rfam、Pfam、Reactome、COG、EggNOG and InterPro annotation;
miRNA-mRNA interaction, lncRNA-mRNA interaction analysis
Protein-Protein Interaction (PPI) analysis
Co-expression Interaction Network Analysis
Custom dataset table upload
Further customization of Bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative.