Long Non-coding RNA Sequencing

Long Non-coding RNA Sequencing

Service Description

Long non-coding RNAs (lncRNAs) are a large class of transcribed RNA molecules with a length of more than 200 nucleotides that do not encode proteins. lncRNAs are thought to encompass nearly 30,000 different transcripts in humans, hence lncRNA transcripts account for the major part of the non-coding transcriptome.

lncRNA discovery is still at a an early stage and only a small proportion of lncRNAs have so far been investigated. Although we can start to classify different types of lncRNA functions, we are still far from being able to predict the function of new lncRNAs.

Sequencing Service Description

BGI offers expression profiling as one way to uncover the function of lncRNA. Identifying lncRNAs that are differentially expressed during development or in particular situations can shed light on their potential functions. Alternatively, looking for lncRNAs and protein-coding genes whose expression is correlated, can indicate co-regulation or related functions.

Project Workflow

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

  • Sample QC
  • Library QC
  • Sequencing QC
  • Data QC
  • Sample Preparation
  • Library Preparation
  • Sequencing
  • Data output
  • Bioinformatics Analysis

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Sequencing Service Specification

DNBSEQ™ Long non-coding RNA Sequencing services are executed with the DNBSEQ™ Technology Platform, with the benefit of lower cost and faster turnaround time.

  • Sample Preparation and Services

    Sample Preparation and Services

    • paired-end 100 bp
    • Strand specific library with rRNA removal
    • Data and bioinformatics analysis are available in standard file formats
    • Advanced RNA data visualization and data mining with Dr.Tom system
  • Sequencing Quality Standard

    Sequencing Quality Standard

    • Guaranteed ≥80% of bases with quality score of ≥Q30
  • Turn Around Time

    Turn Around Time

    • Typical 27 working days from sample QC acceptance to filtered raw data availability
    • Expedited services are available, contact your local BGI specialist for details

Sample Requirements

We can process your total RNA, blood, cell line, FFPE, fresh frozen tissues samples from a variety of species, with the following general requirements.

Purity: OD260/280 ≥ 2.0, OD260/230 ≥ 2.0, without degradation and DNA contamination


RNA Amount and Concentration

RIN Value

Human,mouse and rat

Total RNA ≥200ng,
Concentration ≥20ng/μl

≥ 7.0

Data Analysis

In addition to data output, BGI offers a range of standard and customized bioinformatics pipelines for your Long non-coding RNA sequencing project. Reports and output data files are delivered in industry standard file formats: FASTQ, BAM and Excel.

Standard Analysis

  • Identification of mRNA and LncRNA

  • Quantification and differential expression analysis

  • Gene ontology analysis and pathway enrichment analysis

Dr.Tom system Analysis

  • Transcription factor prediction (For Animal TFDB/Plant TFDB)

  • GSEA Analysis

  • Rfam、Pfam、Reactome、COG、EggNOG and InterPro annotation;

  • miRNA-mRNA interaction, lncRNA-mRNA interaction analysis

  • Protein-Protein Interaction (PPI) analysis

  • Co-expression Interaction Network Analysis

  • Custom dataset table upload

  • Further customization of Bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative.

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