Transcriptome sequencing is used to reveal the presence, quantity and structure of RNA in a biological sample under specific conditions. Compared to hybridization-based RNA quantification methods such as microarray analysis, sequencing-based transcriptome detection can quantify gene expression with low background, high accuracy and high levels of reproducibility within a large dynamic range. In addition, transcriptome sequencing does not require an existing genome sequence and can detect mutations, splice variants and fusion genes that can not be detected by microarrays.
BGI Transcriptome Sequencing services are executed with BGI’s unique DNBseq™ Technology Platform for great sequencing data at the lowest cost in the industry.
DNBseq™ is BGI’s proprietary sequencing technology, developed by our Complete Genomics subsidiary in Silicon Valley.
Give our DNBseq™ Transcriptome Sequencing services a try and find out why so many trust their valuable research samples to the world-class sequencing experience of BGI.
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
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BGI Transcriptome Sequencing services are executed with the DNBseq™ Technology Platform, with the benefit of lower cost and fast turnaround time.
We can process your total RNA, blood, cell line, FFPE, fresh frozen tissues and single cell samples from a variety of species, with the following general requirements.
OD:
OD260/280 ≥1.8
OD260/230 ≥1.8
Human/Animal
Total RNA ≥200ng,
Concentration ≥20ng/μl
RIN≥7.0
28S/18S≥1.0
Fungi
Total RNA ≥1μg,
Concentration ≥40ng/μ
RIN≥6.5
28S/18S≥1.0
Plant
Total RNA ≥1μg,
Concentration ≥40ng/μl
RIN≥6.5
28S/18S≥1.0
Insects
Total RNA ≥1μg,
Concentration ≥40ng/μl
NA
Prokaryotes
Total RNA ≥1μg,
Concentration ≥40ng/μl
RIN≥7.0
23S/16S≥1.0
In addition to raw data output, BGI offers a range of standard and customized bioinformatics pipelines for your transcriptome sequencing project. Reports and output data files are delivered in industry standard file formats: FASTQ, BAM and Excel.
Quantitative expression profiles
Alternative splicing analysis
Fusion gene analysis
SNP and Indel detection
Time series analysis
Gene ontology analysis
Pathway enrichment analysis
Hierarchical clustering analysis
Protein-Protein Interaction (PPI) analysis
Fungal pathogenic gene annotation (For fungi)
Plant disease resistance genes annotation (For plant)
Further customization of Bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative.
