Transcriptome sequencing is used to reveal the presence, quantity and structure of RNA in a biological sample under specific conditions. Compared to hybridization-based RNA quantification methods such as microarray analysis, sequencing-based transcriptome detection can quantify gene expression with low background, high accuracy and high levels of reproducibility within a large dynamic range. In addition, transcriptome sequencing does not require an existing genome sequence and can detect mutations, splice variants and fusion genes that can not be detected by microarrays.
Unique DNBSEQ™ Technology Platform
BGI Transcriptome Sequencing services are executed with BGI’s unique DNBSEQ™ Technology Platform for great sequencing data at the lowest cost in the industry.
DNBSEQ™ is BGI’s proprietary sequencing technology, developed by our Complete Genomics subsidiary in Silicon Valley.
Give our DNBSEQ™ Transcriptome Sequencing services a try and find out why so many trust their valuable research samples to the world-class sequencing experience of BGI.
Applications
Biomarker discovery
Drug development
Evolutionary study
Resistance and adaptability study
Project Workflow
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!
Sequencing Service Specification
BGI Transcriptome Sequencing services are executed with the DNBSEQ™ Technology Platform, with the benefit of lower cost and fast turnaround time.
Sample Preparation and Services
Multiple choices for mRNA enrichment and rRNA removal kits
Stranded and non-stranded sequencing is available
100bp and 150bp paired-end sequencing options available
≥30 Million reads per sample recommended
Raw data and bioinformatics analysis are available in standard file formats
Advanced and custom bioinformatics data analysis
Cloud-based data storage and delivery system
Sequencing
Guaranteed ≥80% of bases with quality score of ≥Q30
Turnaround Time
Typical 18 working days from sample QC acceptance to data availability
Expedited services are available, contact your local BGI specialist for details
Sample Requirements
We can process your total RNA, blood, cell line, FFPE, fresh frozen tissues and single cell samples from a variety of species, with the following general requirements.
OD:
OD260/280 ≥1.8
OD260/230 ≥1.8
SPECIES
RNA AMOUNT AND CONCENTRATION
RIN VALUE
Human/Animal
Total RNA ≥200ng,
Concentration ≥20ng/μl
RIN≥7.0
28S/18S≥1.0
Fungi
Total RNA ≥1μg,
Concentration ≥40ng/μ
RIN≥6.5
28S/18S≥1.0
Plant
Total RNA ≥1μg,
Concentration ≥40ng/μl
RIN≥6.5
28S/18S≥1.0
Insects
Total RNA ≥1μg,
Concentration ≥40ng/μl
NA
Prokaryotes
Total RNA ≥1μg,
Concentration ≥40ng/μl
RIN≥7.0
23S/16S≥1.0
Data Analysis
In addition to raw data output, BGI offers a range of standard and customized bioinformatics pipelines for your transcriptome sequencing project. Reports and output data files are delivered in industry standard file formats: FASTQ, BAM and Excel.