Isobaric Label Quantitation

Service Description

We have developed innovative solutions for optimizing the process of accurately quantifying proteins in complex biological matrices and other complex mixtures.

 

Isobaric Label Quantitative Proteomics

Isobaric Label services incorporate sample multiplexing to provide deep proteome coverage and highly precise quantitation of small or medium-sized sample sets. Samples are prepared and labeled separately, then all samples are pooled and analyzed by nano-flow LC-MS/MS1.

Multiplexed peptides elute as uniform chromatographic peaks and the peptide ions are analyzed using data-dependent selection and high field Orbitrap MS/MS detection. Peptide fragmentation yields specific reporter ions corresponding to each of the multiplexed channels.

We provide Isobaric Label Quantitative Proteomics service using Tandem Mass Tagging (TMT) technology developed by Proteome Sciences plc and licensed by Thermo Fisher Scientific2.

Project Workflow


Individual samples


Pooled samples



Sample preparation

Peptide isobaric labeling

HPLC-UV Fractionation

LC-MS/MS DDA Analysis

Multiplexed quantitation and bioinformatics

References

 

[1]. Wong, J. W. H., & Cagney, G. (2009). An Overview of Label-Free Quantitation Methods in Proteomics by Mass Spectrometry. Proteome Bioinformatics, 273–283.doi:10.1007/978-1-60761-444-9_18.

 

[2]. Kelstrup CD, Bekker-Jensen DB, Arrey TN, Hogrebe A, Harder A, Olsen JV. Performance Evaluation of the Q Exactive HF-X for Shotgun Proteomics. J Proteome Res. 2018 Jan 5;17(1):727-738. doi: 10.1021/acs.jproteome.7b00602.

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Mass Spectrometry Service Specification

Isobaric Label Quantitative Proteomics services are performed using nano-flow liquid chromatography and high resolution Orbitrap mass spectrometry.

  • Sample Preparation and Services

    Sample Preparation and Services

    • Digestion performed using sequencing-grade trypsin or alternative proteolysis
    • Increase proteome depth of coverage using UHPLC-UV sample fractionation
    • Each fraction analyzed using 180 min nano-flow LC-MS/MS using a Q Exactive HF-X Orbitrap mass spectrometer
  • Mass Spec Services Quality Standard

    Mass Spec Services Quality Standard

    • Summary including all methods and data analysis
    • Reports provided in Excel or PDF format, RAW files available upon request
  • Turn Around Time

    Turn Around Time

    • Typical 20 working days from sample QC acceptance to data report delivery

Sample Requirements

We accept protein samples in a variety of formats. For attaining maximum proteome coverage we recommend utilizing our sample fractionation services, performed using our off-line UHPLC-UV platform.

Sample type Amount and Concentration Minimum sample volume
Protein sample in liquid solution Recommended 1000 µg; 2 µg/µL 250 µL
Minimum Required 50 µg; 1 µg/µL 50 µL

Data Analysis

Advanced Bioinformatics

  • Data analysis and validation performed with Biognosys software

  • GO (Gene ontology) category analysis

  • COG (Cluster of Orthologous Groups of proteins) category analysis

  • Pathway analysis

  • DEPs (differentially expressed proteins) cluster analysis

  • DEPs GO enrichment analysis

  • DEPs pathway enrichment analysis

Isobaric Label Quantitative Proteomics Resources