We have developed innovative solutions for optimizing the process of accurately quantifying proteins in complex biological matrices and other complex mixtures.
Isobaric Label TMT Quantitation Proteomics
Isobaric Label TMT services incorporate sample multiplexing to provide deep proteome coverage and highly precise quantitation of small or medium-sized sample sets. Samples are prepared and labeled separately, then all samples are pooled and analyzed by nano-flow LC-MS/MS1.
Multiplexed peptides elute as uniform chromatographic peaks and the peptide ions are analyzed using data-dependent selection and high field Orbitrap MS/MS detection. Peptide fragmentation yields specific reporter ions corresponding to each of the multiplexed channels.
We provide Isobaric Label Quantitative Proteomics service using Tandem Mass Tag (TMT) technology developed by Proteome Sciences plc and licensed by Thermo Fisher Scientific2.
Peptide isobaric labeling
LC-MS/MS DDA Analysis
Multiplexed quantitation and bioinformatics
. Wong, J. W. H., & Cagney, G. (2009). An Overview of Label-Free Quantitation Methods in Proteomics by Mass Spectrometry. Proteome Bioinformatics, 273–283.doi:10.1007/978-1-60761-444-9_18.
. Kelstrup CD, Bekker-Jensen DB, Arrey TN, Hogrebe A, Harder A, Olsen JV. Performance Evaluation of the Q Exactive HF-X for Shotgun Proteomics. J Proteome Res. 2018 Jan 5;17(1):727-738. doi: 10.1021/acs.jproteome.7b00602.