Peptide Mapping

Service Description

BGI provides LC-MS/MS peptide mapping services to support biopharmaceutical and biotechnology applications. Our Peptide Mapping services are designed to characterize and monitor the molecular details of a therapeutic protein drug at each position in the amino acid sequence1,2.

 

Project Workflow

Sequence Verification

We provide amino acid sequence verification services and PTM mapping for purified proteins. Our service is useful for accurately validating expression of recombinant proteins or identifying process related PTMs on biologic drugs.


Individual samples



Gentle sample preparation

LC-UV-MS/MS DDA Analysis

Peptide map data analysis

 

Molecular Assessment

We can help you characterize amino acid sequence liability. We provide a Molecular Assessment service to monitor specific amino acid modifications in response to a forced degradation treatment customized to meet your project needs. Our service is optimized for high dynamic range peptide sequence coverage.


Sample batch

Individual samples




Forced sample degradation series

Gentle sample preparation

LC-UV-MS/MS DDA Analysis

Peptide map data analysis

Molecular Assessment Profile

 

References

 

[1]. Lauber MA, Koza SM, McCall SA, Alden BA, Iraneta PC, Fountain KJ. High-resolution peptide mapping separations with MS-friendly mobile phases and charge-surface-modified C18. Anal Chem. 2013 Jul 16;85(14):6936-44. doi: 10.1021/ac401481z.

 

[2]. Mouchahoir T, Schiel JE. Development of an LC-MS/MS peptide mapping protocol for the NISTmAb. Anal Bioanal Chem. 2018 Mar;410(8):2111-2126. doi: 10.1007/s00216-018-0848-6.

How to Order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Mass Spectrometry Service Specification

Peptide Mapping services are performed using analytical flow liquid chromatography, UV detection, and high resolution Orbitrap mass spectrometry.

  • Sample Preparation and Services

    Sample Preparation and Services

    • Gentle digestion performed using sequencing-grade trypsin or alternative proteolysis designed to minimize sample preparation artifacts
    • In-line UV detection also possible. Optimized for minimal sample oxidation
    • Each fraction analyzed using 150 min analytical flow LC-MS/MS using a Q Exactive HF-X Orbitrap mass spectrometer
    • Data analysis and validation performed with BioPharma Finder software
    • We can work with you to develop customized Multi-Attribute Method (MAM) services using Chromeleon 7 software (21 CFR Part 11 compliant)
  • Mass Spec Services Quality Standard

    Mass Spec Services Quality Standard

    • Summary including all methods and data analysis
    • Reports provided in Excel or PDF format, RAW files available upon request
  • Turn Around Time

    Turn Around Time

    • Typical 20 working days from sample QC acceptance to data report delivery

Sample Requirements

Sample type Amount and Concentration Minimum sample volume
Purified biologic sample in liquid solution Recommended 1000 µg; 10 µg/µL 100 µL
Minimum Required 10 µg; 1 µg/µL 10 µL

Data Analysis

Advanced Bioinformatics

  • Data analysis and validation performed with BioPharma Finder software

  • Peptide mapping method for identifying sequence variants, unknown modification, and PTM co-occupancy

  • Amino acid site PTM status and relative abundance

  • Reports provided in Excel or PDF format, RAW files available upon request