Protein Identification

Service Description

Modern liquid chromatography and mass spectrometry technology provides a means to routinely perform non-targeted protein identification1.

BGI can help simplify the challenge of protein identification for your project.

 

Project Workflow

In-Gel Identification

We can identify proteins isolated in gel bands or spots. Our service includes destaining, in-gel digestion, nano-flow LC-MS/MS, and peptide spectral match data analysis.





In-gel digestion

LC-MS/MS analysis

Data analysis

 

In-Solution Identification

Our in-solution protein identification services can be used for applications such as monitoring recombinant protein expression and affinity purification systems.





In-solution digestion

LC-MS/MS analysis

Data analysis

 

References

 

[1]. Baldwin MA. Protein identification by mass spectrometry: issues to be considered. Mol Cell Proteomics. 2004 Jan;3(1):1-9. Epub 2003 Nov 6. Review. PubMed PMID: 14608001

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Mass Spectrometry Service Specification

Protein Identification services are performed using nano-flow liquid chromatography and high resolution Orbitrap mass spectrometry.

  • Sample Preparation and Services

    Sample Preparation and Services

    • Digestion performed using sequencing-grade trypsin
    • 60 min nano-flow LC-MS/MS using a Q Exactive HF-X Orbitrap mass spectrometer
  • Mass Spec Services Quality Standard

    Mass Spec Services Quality Standard

    • Summary including all methods and data analysis
    • Reports provided in Excel or PDF format, RAW files available upon request
  • Turn Around Time

    Turn Around Time

    • Typical 20 working days from sample QC acceptance to data report delivery

Sample Requirements

We accept protein samples in solution or gel format. For complex in-solution samples we can provide electrophoresis services, including preparative SDS-PAGE and analytical staining.

Sample type Amount and Concentration Minimum sample volume
Protein sample in liquid solution or gel band/spot Recommended 100 µg; 2 µg/µL or above 10 µL

Data Analysis

Advanced Bioinformatics

  • Data analysis and validation performed with SEQUEST or Mascot

  • Top protein identifications, peptide spectral match count, and sequence coverage provided

  • Up to 30 proteins ranked by peptide spectral match count

Protein Identification Resources

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