Sample Preparation and Services
- Digestion performed using sequencing-grade trypsin
- 60 min nano-flow LC-MS/MS using a Q Exactive HF-X Orbitrap mass spectrometer
Modern liquid chromatography and mass spectrometry technology provides a means to routinely perform non-targeted protein identification1.
BGI can help simplify the challenge of protein identification for your project.
We can identify proteins isolated in gel bands or spots. Our service includes destaining, in-gel digestion, nano-flow LC-MS/MS, and peptide spectral match data analysis.
Our in-solution protein identification services can be used for applications such as monitoring recombinant protein expression and affinity purification systems.
Protein Identification services are performed using nano-flow liquid chromatography and high resolution Orbitrap mass spectrometry.
We accept protein samples in solution or gel format. For complex in-solution samples we can provide electrophoresis services, including preparative SDS-PAGE and analytical staining.
|Sample type||Amount and Concentration||Minimum sample volume|
|Protein sample in liquid solution or gel band/spot||Recommended||100 µg; 2 µg/µL or above||10 µL|
Data analysis and validation performed with SEQUEST or Mascot
Top protein identifications, peptide spectral match count, and sequence coverage provided
Up to 30 proteins ranked by peptide spectral match count