Quantitative Proteomics

Quantitative Proteomics

Quantitative Proteomic Services

BGI supports multiple workflows for proteomic quantitation. We have developed innovative solutions for optimizing the process of accurately quantifying proteins in complex biological matrices and other complex mixtures. Our quantitative services include Label-Free Data-Independent Acquisition (DIA), Isobaric Label (TMT tag), or Targeted Quantitation.

 

We are happy to work with you to determine the best quantitative proteomics service for your project1.

 

 

 

Label-Free DIA

Data-Independent Acquisition (DIA) is a label-free quantitative technique which provides highly consistent quantitation and broad proteome coverage. This service references a customized spectral library which is constructed using your specific sample and can be updated and grown over a period of time2.

 

Label-Free DIA Quantitative Proteomics service is ideal for projects with large or expanding sample sets which require accurate and reproducible quantitation.

 

Isobaric Label

Isobaric Label services incorporate sample multiplexing to provide deep proteome coverage and highly precise quantitation of small or medium-sized sample sets.

 

Samples are prepared and labeled separately, then all samples are pooled and analyzed by nano-flow LC-MS/MS. Labeled-peptides elute as a single chromatographic peaks and are ionized peptides are analyzed using data-dependent selection and high-field Orbitrap MS/MS detection. The multiplexed labeled peptides yield specific reporter ions corresponding to each of the multiplexed channels.

 

We provide Isobaric Label Quantitative Proteomics service using Tandem Mass Tagging (TMT) technology developed by Proteome Sciences plc and licensed by Thermo Fisher Scientific3.

 

Targeted PRM

Parallel Reaction Monitoring (PRM) technology provides a cost-effective, high-bandwidth alternative to Selected/Multiple Reaction Monitoring (SRM, MRM)4. Targeted PRM Quantitation is our most sensitive and most selective quantitation service. Our laboratory utilizes high resolution Orbitrap MS/MS; and combines scanning of both peptides and the peptide fragments.

References

 

[1].  Wong, J. W. H., & Cagney, G. (2009). An Overview of Label-Free Quantitation Methods in Proteomics by Mass Spectrometry. Proteome Bioinformatics, 273–283.doi:10.1007/978-1-60761-444-9_18.

 

[2]. Searle BC, Pino LK, Egertson JD, Ting YS, Lawrence RT, MacLean BX, Villén J, MacCoss MJ. Chromatogram libraries improve peptide detection and quantification by data independent acquisition mass spectrometry. Nat Commun. 2018 Dec 3;9(1):5128. doi: 10.1038/s41467-018-07454-w.

 

[3]. Kelstrup CD, Bekker-Jensen DB, Arrey TN, Hogrebe A, Harder A, Olsen JV. Performance Evaluation of the Q Exactive HF-X for Shotgun Proteomics. J Proteome Res. 2018 Jan 5;17(1):727-738. doi: 10.1021/acs.jproteome.7b00602.

 

[4]. Bourmaud A, Gallien S, Domon B. Parallel reaction monitoring using quadrupole-Orbitrap mass spectrometer: Principle and applications. Proteomics. 2016 Aug;16(15-16):2146-59. doi: 10.1002/pmic.201500543.