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Targeted PRM Quantitation

Service Description

We have developed innovative solutions for optimizing the process of accurately quantifying proteins in complex biological matrices and other complex mixtures.

 

Targeted PRM Quantitation

Targeted PRM is the most sensitive and specific method for peptide quantitation. This service is ideal for screening large numbers of samples for biomarker proteins and post-translational modification sites1.

Parallel Reaction Monitoring (PRM) technology is a cost-effective, high-bandwidth alternative to Selected/Multiple Reaction Monitoring (SRM/MRM)2. Our service utilizes two stages of high resolution Orbitrap MS detection, scanning for both signature peptides (Full MS) as well as peptide fragment reporter ions (MS/MS).

Project Workflow

Sample preparation
Targeted LC-MS/MS analysis
Peptide quantitation

References

 

[1]. Wong, J. W. H., & Cagney, G. (2009). An Overview of Label-Free Quantitation Methods in Proteomics by Mass Spectrometry. Proteome Bioinformatics, 273–283.doi:10.1007/978-1-60761-444-9_18.

 

[2]. Bourmaud A, Gallien S, Domon B. Parallel reaction monitoring using quadrupole-Orbitrap mass spectrometer: Principle and applications. Proteomics. 2016 Aug;16(15-16):2146-59. doi: 10.1002/pmic.201500543.

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Mass Spectrometry Service Specification

Targeted PRM Peptide Quantitation service is performed using nano-flow liquid chromatography and high resolution Orbitrap mass spectrometry. For high-throughput applications we can provide this service using micro-flow or analytical flow rates.

  • Sequencing Service Specification

    Sequencing Service Specification

    • Sample digestion performed using sequencing-grade trypsin or alternative proteolysis
  • Mass Spec Services Quality Standard

    Mass Spec Services Quality Standard

    • Summary including all methods and data analysis
    • Reports provided in Excel or PDF format, RAW files available upon request
  • Turn Around Time

    Turn Around Time

    • Typical 20 working days from sample QC acceptance to data report delivery

Sample Requirements

We accept protein samples in a variety of formats. For attaining maximum proteome coverage we recommend utilizing our sample fractionation services, performed using our off-line UHPLC-UV platform.

Sample type Minimum sample volume
Protein sample in liquid solution
10 µL

Data Analysis

We can adapt our Targeted PRM peptide quantitation services to meet your requirements using multiple data analysis platforms, including Skyline, Chromeleon, or Qual Browser.