We have developed innovative solutions for optimizing the process of accurately quantifying proteins in complex biological matrices and other complex mixtures.

Service Description

Targeted PRM is the most sensitive and specific method for peptide quantitation. This service is ideal for screening large numbers of samples for biomarker proteins and post-translational modification sites1.

Parallel Reaction Monitoring (PRM) technology is a cost-effective, high-bandwidth alternative to Selected/Multiple Reaction Monitoring (SRM/MRM)2. Our service utilizes two stages of high resolution Orbitrap MS detection, scanning for both signature peptides (Full MS) as well as peptide fragment reporter ions (MS/MS).

Targeted PRM Quantitation

Project Workflow

[1]. Wong, J. W. H., & Cagney, G. (2009). An Overview of Label-Free Quantitation Methods in Proteomics by Mass Spectrometry. Proteome Bioinformatics, 273–283.doi:10.1007/978-1-60761-444-9_18. 

[2]. Bourmaud A, Gallien S, Domon B. Parallel reaction monitoring using quadrupole-Orbitrap mass spectrometer: Principle and applications. Proteomics. 2016 Aug;16(15-16):2146-59. doi: 10.1002/pmic.201500543.

References

How to order

Targeted PRM Peptide Quantitation service is performed using nano-flow liquid chromatography and high resolution Orbitrap mass spectrometry. For high-throughput applications we can provide this service using micro-flow or analytical flow rates.

<ul><li>Sample digestion performed using sequencing-grade trypsin or alternative proteolysis</li></ul>

 Sequencing Service Specification

<ul><li>Summary including all methods and data analysis</li><li>Reports provided in Excel or PDF format, RAW files available upon request</li></ul>

 Mass Spec Services Quality Standard

<ul><li>Typical 20 working days from sample QC acceptance to data report delivery</li></ul>

Turn Around Time

Mass Spectrometry Service Specification

We accept protein samples in a variety of formats. For attaining maximum proteome coverage we recommend utilizing our sample fractionation services, performed using our off-line UHPLC-UV platform.

Sample Requirements

We can adapt our Targeted PRM peptide quantitation services to meet your requirements using multiple data analysis platforms, including Skyline, Chromeleon, or Qual Browser.

Data Analysis

Targeted PRM Quantitative Proteomics Resources

BGI Americas have developed innovative solutions for optimizing the process of comprehensive qualitative and quantitative analysis of all endogenous peptides.