Sequencing Service Specification
- Sample digestion performed using sequencing-grade trypsin or alternative proteolysis
We have developed innovative solutions for optimizing the process of accurately quantifying proteins in complex biological matrices and other complex mixtures.
Targeted PRM is the most sensitive and specific method for peptide quantitation. This service is ideal for screening large numbers of samples for biomarker proteins and post-translational modification sites1.
Parallel Reaction Monitoring (PRM) technology is a cost-effective, high-bandwidth alternative to Selected/Multiple Reaction Monitoring (SRM/MRM)2. Our service utilizes two stages of high resolution Orbitrap MS detection, scanning for both signature peptides (Full MS) as well as peptide fragment reporter ions (MS/MS).
Targeted PRM Peptide Quantitation service is performed using nano-flow liquid chromatography and high resolution Orbitrap mass spectrometry. For high-throughput applications we can provide this service using micro-flow or analytical flow rates.
We accept protein samples in a variety of formats. For attaining maximum proteome coverage we recommend utilizing our sample fractionation services, performed using our off-line UHPLC-UV platform.
|Sample type||Minimum sample volume|
|Protein sample in liquid solution|
We can adapt our Targeted PRM peptide quantitation services to meet your requirements using multiple data analysis platforms, including Skyline, Chromeleon, or Qual Browser.