High-Res HLA Typing Service

High-Res HLA Typing Service

Service Description

The Human Leukocyte Antigen (HLA) system or complex is a gene complex encoding the major histocompatibility complex (MHC) proteins in humans. These cell-surface proteins are responsible for the regulation of the immune system in humans. HLAs have been showed to play an important role in disease defense, to protect against cancers and are the major cause of organ transplant rejections. HLA can also present biomarkers of susceptibility to certain disease or adverse drug reactions.

The HLA gene complex resides on a 3Mbp stretch of chromosome 6p21, which is highly polymorphic and poses significant challenges in investigating the role of HLA genetic variations in diseases.

BGI has developed a dedicated sequencing-based solution to deliver precise and high-resolution HLA-typing result to meet your research needs. This solution is cleared by cFDA in China for clinical application, but is intended for research use only elsewhere.

 

Why BGI for HLA typing

  • High resolution: Employs the international gold standard method to provide HLA typing at 4-digit resolution.
  • Accurate: 100% accuracy achieved in 25 consecutive external quality assessments from the UCLA International HLA DNA Exchange program.
  • Multiple and flexible testing choices: Testing sites including HLA-A, B, C, G, DQB1, DRB1, DQA1, DPA1, DPB1, DRB3, DRB4, DRB5 to meet your project requirements.
  • Quality Standards: NGS High-Resolution HLA Typing services are performed under strict quality systems. BGI is a China Marrow Donor Program (CMDP) contract laboratory.

 

High Resolution HLA Typing for Research Applications

  • Applications include Blood disease, immune disease, drug allergy and others.
  • Compatibility assessment for:
  1. Recipients and donors for allogeneic stem cell transplantation.
  2. Recipients and donors for organ transplantation.

 

Project Workflow

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

We have established HLA typing workflows for both NGS and Sanger sequencing platforms:

 

BGI HLA workflow

Service Specification

Testing sites include HLA-A, B, C, DQB1, DRB1, DQA1, DPA1, DPB1, DRB3, DRB4, DRB5, G. You can choose testing site or site combinations according to your research needs.

  • Sample Types

    Sample Types

    • Supported sample types: Whole blood, gDNA, mouth swabs.
  • Turn Around Time

    Turn Around Time

    • Typical 25 working days from sample receipt to result delivery, depending on project parameters. Expedited services are available, contact your local BGI specialist for details.

Data Analysis

BGI offers a range of standard and customized bioinformatic pipelines for your project reports and output data files are delivered in industry-standard file formats.

Standard Analysis

  • Data filtering

  • Fragment assembling (NGS data)

  • Alignment to IMGT/HLA database

  • HLA typing at 4-digit resolution

Advanced Analysis

Association analysis between phenotype and HLA types

Sample Requirements

We can process your Whole blood, gDNA, and Mouth swab samples, with the following general requirements:

Sample Type

Requirements

Whole Blood

4-8 ml, using EDTA anticoagulant tube

gDNA

Concentration ≧30 ng/μl,
Total Mass ≥ 5μg,
OD260/280: 1.8-2.0

Mouth Swabs

2 swabs from each side of the cheek mucosa, 4 in total,
wiping about thirty times for each swab