- Supported sample types: Whole blood, gDNA, mouth swabs.
The Human Leukocyte Antigen (HLA) system or complex is a gene complex encoding the major histocompatibility complex (MHC) proteins in humans. These cell-surface proteins are responsible for the regulation of the immune system in humans. HLAs have been showed to play an important role in disease defense, to protect against cancers and are the major cause of organ transplant rejections. HLA can also present biomarkers of susceptibility to certain disease or adverse drug reactions.
The HLA gene complex resides on a 3Mbp stretch of chromosome 6p21, which is highly polymorphic and poses significant challenges in investigating the role of HLA genetic variations in diseases.
BGI has developed a dedicated sequencing-based solution to deliver precise and high-resolution HLA-typing result to meet your research needs. This solution is cleared by cFDA in China for clinical application, but is intended for research use only elsewhere.
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
We have established HLA typing workflows for both NGS and Sanger sequencing platforms:
Testing sites include HLA-A, B, C, DQB1, DRB1, DQA1, DPA1, DPB1, DRB3, DRB4, DRB5, G. You can choose testing site or site combinations according to your research needs.
BGI offers a range of standard and customized bioinformatic pipelines for your project reports and output data files are delivered in industry-standard file formats.
Fragment assembling (NGS data)
Alignment to IMGT/HLA database
HLA typing at 4-digit resolution
Association analysis between phenotype and HLA types
We can process your Whole blood, gDNA, and Mouth swab samples, with the following general requirements:
4-8 ml, using EDTA anticoagulant tube
Concentration ≧30 ng/μl,
Total Mass ≥ 5μg,
2 swabs from each side of the cheek mucosa, 4 in total,
wiping about thirty times for each swab