Oseq™ Solid Tumor Panels

Oseq™ Solid Tumor Panels

Service Description

Human cancers typically carry several types of genomic variations, such as Single Nucleotide Variants (SNV), Copy Number Variations (CNV), Insertions and Deletions (InDels), Structural Variants (SV), and Gene Fusions. These genetic alterations infer important insights for the discovery and development of biomarkers and for use in basic cancer research, patient stratification and clinical research.

BGI has developed multiple comprehensive and specific cancer panels based on many years of cancer research experience and proprietary database generation. These NGS panels are all capture-based, designed to detect all types of genomic tumor alterations, including base substitutions, insertions and deletions, copy number alterations, and selected gene fusions.

For example, our most comprehensive OseqTM Solid Cancer Panel includes 1,053 genes that are strongly supported as clinically actionable cancer genes, with high frequency alterations in the COSMIC database, or play key roles in core cancer pathways.

 

OseqTM Solid Tumor Panels

Multiple OseqTM panels are available for solid tumor research. The OseqTM T for tissue biopsy and OseqTM ctDNA, and OseqTM lung panels are approved clinical test in China. All the panels are for Research use only outside of China.

OseqTM panels are designed for the identification of clinically actionable mutations, as well as discovery of novel variants with important functions, including, but not limited to Lung Cancer, Colorectal Cancer, Breast Cancer, Ovarian Cancer, Melanoma, and Lymphoma.

 

Why OseqTM Solid Cancer Panels

 

Solid Cancer Panel Applications

  • Drug target discovery
  • Biomarker discovery
  • Patient Stratification
  • Understanding of drug response
  • Understanding of drug resistance

Project Workflow

Under a 21CFR Part 11 compliant LIMS system, your samples are tracked from sample accessioning through data reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of the results.

  • Sample QC
  • Library QC
  • Sequencing QC
  • Data QC
  • Sample Preparation
  • Library Preparation
  • Sequencing
  • Raw data output
  • Bioinformatics Analysis

How to Order

Request a quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Sequencing Service Specification

BGI solid cancer panel sequencing services are executed with the Illumina HiSeq sequencing system, in our CAP and CLIA certified service laboratory in Hong Kong. For samples from China, we offer services from Chinese clinical laboratories in mainland China, with our cFDA cleared BGISEQ-500 sequencing platform.

  • Sample Preparation and Services

    Sample Preparation and Services

    • 100bp and 150bp paired-end sequencing options available
    • NimbleGen SeqCap or KAPA Hyper Prep kit for library construction and enrichment
    • Raw data and bioinformatics analysis are available in standard file formats
    • Advanced and custom bioinformatics data analysis available
    • Cloud-based data storage and delivery system
  • Sequencing Quality Standard

    Sequencing Quality Standard

    • Guaranteed ≥ 80% of bases with quality score of ≥ Q30
    • Standard sequencing coverage ≥ 400X is recommended for cancer samples
  • Turn Around Time

    Turn Around Time

    • Typical 30 working days from sample QC acceptance to filtered raw data availability for research.
    • Expedited services are available, including 10-day service for prospective studies. Contact your local BGI specialist for details.

Data Analysis

Raw data and bioinformatics analysis are delivered in standard file formats.

Oseq Data Analysis

  • Filtering

  • Alignment

  • SNP calling and annotation

  • SNP validation and comparison

  • SNP functionality and conservation prediction

  • InDel calling and annotation

  • CNV calling and annotation

  • Gene fusion calling and annotation

Sample Requirements

We can process your gDNA, Blood, Cell line, Fresh frozen tissue, FFPE samples with the following general requirements:

SAMPLE TYPE

SAMPLE REQUIREMENT

SPECIFICATION

Whole blood control

1-2ml

using EDTA anticoagulant tube

Whole blood

10ml

using Streck cfDNA Tube

DNA

Total Mass ≥ 200ng,
Concentration ≥20 ng/µl

OD260/280: 1.8-2.0

FF

0.5g

OD260/280: 1.8-2.0

FFPE

5 slides

100 mm2, 5-10 µm thick