Sample Submission and Shipping Guidelines

DNA Sample Requirement General Guidelines for:

  • HISEQ Platform Based Services
  • DNBseq™ Based Services
  • PACBIO Platform Based Services

For questions on submitting other sample types or for custom sequencing solutions, please contact our Project Management teams or your BGI Sales Representative to discuss further.
Contact Support: info@bgi-international.com

For HISEQ Platform Based Services

Whole Genome Resequencing ≤800bp normal library (Human/Plant/Animal/Fungal/Bacterial/Metagenomic)

  • Sample type: genomic DNA/PCR product

  • Quality requirements: >1µg, >12.5ng/µl

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

De Novo Genome sequencing ≤800bp normal library (Human/Plant/Animal/Fungal/Bacterial/Metagenomic)

  • Sample type: genomic DNA/PCR product

  • Quality requirements: >1.5µg, >20ng/µl

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

De Novo Genome sequencing 2kb≤X≤6kb (Human/Plant/Animal/Fungal/Bacterial/Metagenomic)

  • Sample type: genomic DNA/PCR product

  • Quality requirements: m>20µg, c>110ng/µl, no degradation or slight degradation

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

De Novo Genome sequencing 8kb≤X≤10kb (Human/Plant/Animal/Fungal/Bacterial/Metagenomic)

  • Sample type: genomic DNA

  • Quality requirements: m>30µg, c>110ng/µl, no degradation or slight degradation

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

De novo Genome sequencing 20kb (Human/Plant/Animal/Fungal/Bacterial/Metagenomic)

  • Sample type: genomic DNA

  • Quality requirements: m>40µg, c>110ng/µl, no degradation or slight degradation

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

De novo Genome sequencing 40kb (human/plant/animal/Fungal/Bacterial/Metagenomic)

  • Sample type: genomic DNA

  • Quality requirements: m>50µg, c>110ng/µl, no degradation or slight degradation

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

De novo Genome sequencing ≤800bp PCR free library (human/plant/animal/Fungal/Bacterial/Metagenomic)

  • Sample type: genomic DNA

  • Quality requirements: m>10µg, c>30ng/µl, no degradation or slight degradation

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice.

Whole Exome Sequencing (human/Animal): GENOMIC DNA

  • Quality requirements: m>1µg, c>12.5ng/µl, no degradation or slight degradation

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice.

Whole Exome Sequencing (human/Animal): FFPE Genomic DNA

  • Quality requirements: m>1µg, c>12.5ng/µl, main fragment size ≥250bp

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

Whole Exome Sequencing (Plant)

  • Sample type: genomic DNA

  • Quality requirements: m>10µg, c>30ng/µl, no degradation or slight degradation

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

Target Region Sequencing: Genomic DNA

  • Quality requirements: m>1µg, c>12.5ng/µl, no degradation or slight degradation

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

Target Region Sequencing: FFPE Genomic DNA

  • Quality requirements: m>1µg, c>12.5ng/µl, main fragment size ≥250bp

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

ChIP Sequencing

  • Sample type: ChIP-ed DNA

  • Quality requirements: m>10ng, c>1ng/µl, Major Peak in 100bp-500bp (Agilent 2100)

  • Sample volume for QC: 1ul for Qubit, 2ul for Agilent 2100

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

RAD- Sequencing

  • Sample type: genomic DNA

  • Quality requirements: m>300ng, c>10ng/µl, no degradation or slight degradation, No RNA and Protein contamination

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

Genotyping by Sequencing

  • Sample type: genomic DNA

  • Quality requirements: m>1µg, c>20ng/µl, no degradation or slight degradation, No RNA and Protein contamination

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

Target region Bisulfite Sequencing (TBS)

  • Sample type: genomic DNA

  • Quality requirements: m>3µg, c>37.5ng/µl, no degradation or slight degradation, No RNA and Protein contamination

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

Whole Genome Bisulfite Sequencing

  • Sample type: genomic DNA

  • Quality requirements: m>1µg, c>15ng/µl, no degradation or slight degradation

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

Meta rDNA Amplicon Sequencing

  • Sample type: genomic DNA

  • Quality requirements: m>1µg, c>20ng/µl, no degradation or slight degradation

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

For DNBseq sequencing technology Based Services

ChIP Sequencing

  • Sample type: ChIP-ed DNA

  • Quality requirements: m>10ng, c>1ng/µl, Major Peak in 100bp-500bp (Agilent 2100)

  • Sample volume for QC: 1ul for Qubit, 2ul for Agilent 2100

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

Whole Exome Sequencing

  • Sample type: genomic DNA

  • Quality requirements: m>1µg, c>12.5ng/µl, No degradation or slight degradation

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

Whole Genome Sequencing

  • Sample type: genomic DNA

  • Quality requirements: m>1µg, c>12.5ng/µl, No degradation or slight degradation

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Re-suspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

For PACBIO Platform Based Services

Pacbio DNA Sequencing (20Kb)

  • Sample type: genomic DNA

  • Quality requirements: m>10.2ug, c>80ng/µl, no degradation or slight degradation

  • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

Pacbio DNA Sequencing (10Kb)

  • Sample type: genomic DNA

  • Quality requirements: m>10.2µg, c>40ng/µl, No degradation or slight degradation

  • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

Pacbio DNA Sequencing (5Kb)

  • Sample type: genomic DNA

  • Quality requirements: m>4.2µg, c>50ng/µl, No degradation or slight degradation

  • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

Pacbio DNA Sequencing (2Kb)

  • Sample type: genomic DNA

  • Quality requirements: m>1.7µg, c>50ng/µl, No degradation or slight degradation

  • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

Pacbio DNA Sequencing (1Kb)

  • Sample type: genomic DNA

  • Quality requirements: m>1.2µg, c>40ng/µl, no degradation or slight degradation

  • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

Pacbio DNA Sequencing (200bp/500bp)

  • Sample type: genomic DNA

  • Quality requirements: m>0.52µg, c>20ng/µl, no degradation or slight degradation

  • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5

  • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

  • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

  • Shipment: Dry ice

RNA Sample Requirement General Guidelines for:

  • HISEQ Platform Based Services
  • DNBseq™ Based Services

For questions on submitting other sample types or for custom sequencing solutions, please contact our Project Management teams or your BGI Sales Representative to discuss further. Contact Support: info@bgi-international.com

For HISEQ Platform Based Services

Standard/Strand-Specific Transcriptome Sequencing (Human/Mouse): Total RNA

  • Quality requirements: m>200ng, c>20ng/µl, RIN≥7.0 (Agilent2100 or equivalent)

  • Sample volume for QC: 2~3ul

  • Resuspension Buffer: Nuclease-free water, EB

  • Shipment: Dry ice

Standard/Strand-Specific Transcriptome Sequencing (Human/Mouse): FFPE RNA

  • Quality requirements: m>200ng, c>70ng/µl, DV200≥30%, RIN≥2.0 (Agilent2100 or equivalent)

  • Sample volume for QC: 2~3ul

  • Resuspension Buffer: Nuclease-free water, EB

  • Shipment: Dry ice

Standard/Strand-Specific Transcriptome Sequencing (Animals/Plants/Microorganisms)

  • Sample type: Total RNA

  • Quality requirements: m>1ug, c>20ng/µl, RIN≥7.0, 28s/18s≥ 1.0(23s/16s≥ 1.0) (Agilent2100 or equivalent)

  • Sample volume for QC: 2~3ul

  • Resuspension Buffer: Nuclease-free water, EB

  • Shipment: Dry ice

Lnc RNA Sequencing (Human/Mouse)

  • Sample type: Total RNA

  • Quality requirements: m>200ng, c>20ng/µl, RIN≥7.0, 28s/18s≥ 1.0 (Agilent2100 or equivalent)

  • Sample volume for QC: 2~3ul

  • Resuspension Buffer: Nuclease-free water, EB

  • Shipment: Dry ice

Lnc RNA Sequencing (Animals/Plants/Microorganisms)

  • Sample type: Total RNA

  • Quality requirements: m>1ug, c>20ng/µl, RIN≥7.0, 28s/18s≥ 1.0 (Agilent2100 or equivalent)

  • Sample volume for QC: 2~3ul

  • Resuspension Buffer: Nuclease-free water, EB

  • Shipment: Dry ice

For DNBseq sequencing technology Based Services

STANDARD/STRAND-SPECIFIC TRANSCRIPTOME SEQUENCING (HUMAN/MOUSE)

  • Sample type: Total RNA

  • Quality requirements: m>200ng, c>20ng/µl, RIN≥7.0, 28s/18s≥1.0 (Agilent2100 or equivalent)

  • Sample volume for QC: 2~3µl

  • Resuspension Buffer: Nuclease-free water, EB

  • Shipment: Dry ice

STANDARD/STRAND-SPECIFIC TRANSCRIPTOME SEQUENCING (ANIMALS/PLANTS/MICROORGANISMS)

  • Sample type: Total RNA

  • Quality requirements: m>1µg, c>40ng/µl, RIN≥6.5(Plant/Fungi), RIN≥7(Other animal), 28S/18S≥1.0 (Agilent2100 or equivalent)

  • Sample volume for QC: 2~3µl

  • Resuspension Buffer: Nuclease-free water, EB

  • Shipment: Dry ice

RNA-Seq (Quantification) (Human/ Mouse)

  • Sample type: Total RNA

  • Quality requirements: m>200ng, c>20ng/µl, RIN≥7.0, 28s/18s≥ 1.0 (Agilent2100 or equivalent)

  • Sample volume for QC: 2~3ul

  • Resuspension Buffer: Nuclease-free water, EB

  • Shipment: Dry ice

RNA-Seq (Quantification) (Animals/Plants/Microorganisms)

  • Sample type: Total RNA

  • Quality requirements: m>1ug, c>40ng/µl, RIN≥7.0, 28s/18s≥ 1.0 (Agilent2100 or equivalent)

  • Sample volume for QC: 2~3ul

  • Resuspension Buffer: Nuclease-free water, EB

  • Shipment: Dry ice.

Small RNA Sequencing: Total RNA

  • Quality requirements: m>1ug, c>50ng/µl, RIN≥8.0, 28s/18s≥1.5(23s/16s≥1.5) (Agilent2100 or equivalent)

  • Sample volume for QC: 2~3ul

  • Resuspension Buffer: Nuclease-free water, EB

  • Shipment: Dry ice

Small RNA Sequencing: Small RNA (<200nt)

  • Quality requirements: m>100ng, c>5ng/µl, (Agilent2100 or equivalent)

  • Sample volume for QC: 2~3ul

  • Resuspension Buffer: Nuclease-free water, EB

  • Shipment: Dry ice

Extraction Requirements

All extractions are performed to the best of BGI’s ability. The customer is charged for work performed regardless of outcome unless otherwise stated. Additional charges for procurement of kits may apply for select extractions.

FFPE samples (DNA and RNA)

  • Recommended Quantity: DNA≥10 (RNA≥5) FFPE slides

  • Thickness: 5µm-10 µm

  • Diameter: 15 mm×20mm

  • Shipment: submitted at room temperature/blue ice

Cell pellets

  • Recommended Quantity: 106 cells

  • BGI does not provide cell culturing

  • Shipment: submitted on dry ice

Fresh Frozen Tissue

  • Recommended Quantity (DNA Extraction): ≥300mg (for DNA Insert >2kb, Recommended Quantity ≥1g)

  • Recommended Quantity (RNA Extraction): ≥30mg

  • Shipment: submitted on dry ice

Whole Blood

  • Recommended Volume (DNA Extraction): ≥1 mL (EDTA Tube)

  • Recommended Volume (RNA Extraction): 2.5 mL (whole blood, PAXgene Blood RNA Tube)

  • Shipment: Fresh submitted on Blue Ice, Frozen submitted on Dry Ice

Plasma/Serum

  • Recommended Volume (DNA Extraction): ≥5 mL

  • Recommended Volume (RNA Extraction): ≥1 mL

  • Shipment: Dry Ice

Stool

  • Recommended Quantity: 50~100 mg (Solid)

  • Recommended Volume: 500uL (OMNIgene•GUT(OMR-200), DNA Genotek Collection tube)

  • Shipment: Solid submitted on dry ice, Collection tube submitted at room temperature/blue ice

Soil

  • Recommended Quantity: 50~150mg

  • Shipment: submitted on dry ice

Swab

  • Recommended Quantity: 2 tubes/sample, 1 swap/tube

  • Shipment: submitted on dry ice

BGI Sample Quality Control Assessment

BGI performs QC free of charge.

DNA / RNA

Pre-Made Libraries

Agilent 2100 BioAnalyzer

RNA

Microplate Reader/Qubit

DNA

Gel Electrophoresis

DNA

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