Low Throughput Single Cell Sequencing is used for the study of heterogeneous samples, rare cell types, cell lineage relationships, and disease evolution. We offer high quality, affordable single cell services, with world-class support from our highly experienced single cell project experts.
Low Throughput Single Cell Sequencing is commonly applied to solve problems associated with the study of heterogeneous samples, rare cell types, cell lineage relationships, and disease evolution. By using deep sequencing of DNA and RNA from a single cell, cellular functions of individual cells can be investigated in great detail.
BGI applies the superior method of Multiple Displacement Amplification (MDA) for single cell genomic DNA amplification (WGS/WES), and Switching Mechanism at 5′ End of RNA Template (SMART-seq2) for single cell RNA amplification (transcriptome/RNA quantification). Contact us with your project requirements, our experts are here to help.
Sample Requirements
DNA
Single Cell: 1-2 cells - 4μl PBS buffer
A Few Cells: 2-1000 cells - 4μl PBS buffer
Amplified single cell gDNA/cDNA:
Concentration: >20ng/ μl, Quantity: >3μg
RNA
Single Cell: 1-2 cells - 4μl lysis buffer
A Few Cells: 2-1000 cells - 4μl lysis buffer
Amplified single cell gDNA/cDNA:
Concentration: >0.2ng/μl, Quantity: >1ng
Total RNA for low input library:
Concentration: >50pg/μl, Quantity: >2ng;
RNA 28S/18S ≥1，RIN ≥7
Sequencing Standards
PE100 sequencing option, depending on your application.
Guaranteed ≥90% of bases with quality score of ≥Q20.
Cell sorting by mouth-controlled microcapillary pipetting system.
Key Service Details
• Typical 40 working days from sample QC acceptance to filtered raw data availability.
• Expedited services are available, contact your local BGI specialist for details.
• Reports and output data files are delivered in industry standard file formats: BAM, .xls, .png and FASTQ data.
BGI's Single Cell Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ is a proprietary sequencing technology, first developed by MGI, and can offer advantages in terms of lower amplification error rates and much lower duplication rates for some sequencing applications. In addition, studies have shown the index hopping rate in DNBSEQ™ platforms can be much lower when compared to that of other platforms.
Contact us with any general enquiries or questions for our sequencing experts, we are here to help.
Access Further Sequencing Resources
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results. Access more sequencing resources below for full access to our range of educational resources and sample guides.
Opening a BGI Customer Account enables you to request a quote and order services from us. It also provides access to our range of educational resources including educational webinars providing practical sequencing tips and our complete sample preparation guides.