For many applications, Whole Exome Sequencing is gaining popularity as a viable and cost-effective alternative for Whole Genome Sequencing. BGI has performed professional sequencing services for many years at multiple locations globally, to support biomedical research and to benefit small and large-scale clinical trials and other projects in pharmaceutical drug development.
Besides raw sequencing data output, BGI offers standard and custom bioinformatics services to suit your specific research needs.
Unique DNBseq Technology
BGI Whole Exome Sequencing services are executed with the Illumina sequencing system, or exclusively with DNBseq sequencing technology, for great sequencing data at the lowest cost in the industry.
DNBseq is a high-throughput sequencing platform developed by a subsidiary of BGI, Complete Genomics, in Silicon Valley. This system is powered by combinatorial Probe-Anchor Synthesis (cPAS), linear isothermal Rolling-Circle Replication and DNA Nanoballs (DNBTM) technology, followed by high-resolution digital imaging.
Ask your BGI representative about our current introductory pricing for Whole Exome Sequencing with the BGI’s own DNBseq technology.
Applications
Germline variant detection
Somatic variant detection
Associating DNA variants with a phenotype, such as a disease
Structural variant discovery
Copy number variation
Discovering biomarkers and therapeutic targets
Project Workflow
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
Sample QC
Library QC
Data QC
Sample Preparation
Exome Capture Library Preparation
Sequencing
Raw Data Output
Bioinformatics Analysis
How to order
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!
Sequencing Service Specification
BGI Whole Exome Sequencing services are executed with DNBseq sequencing technology and Illumina sequencing system.
Sample Preparation and Services
100bp and 150bp paired-end sequencing options available
Agilent SureSelect kit, BGI kit, NimbleGen SeqCap or KAPA Hyper Prep kit for library construction and enrichment
Raw data and bioinformatics analysis are available in standard file formats
Advanced and custom bioinformatics data analysis available
Cloud-based data storage and delivery system
Sequencing Quality Standard
Guaranteed ≥ 80% of bases with quality score of ≥Q30
Standard sequencing coverage ≥50X; ≥150X is recommended for cancer samples
Turn Around Time
Typical 40 working days from sample QC acceptance to filtered raw data availability.
Expedited services are available, contact your local BGI specialist for details
Sample Requirements
We can process your gDNA, Blood, Cell line, Fresh frozen tissue, FFPE samples and single cell applications from human and rodents, with the following general requirements:
DNA concentration
Minimum sample volume
Regular Samples
Intact genomic DNA ≥ 1µg, Concentration ≥ 12.5 ng/ µl
15 μl
Low Input Samples
Genomic DNA ≥ 200 ng, Concentration ≥ 2.5 ng/ µl
15 μl
Data Analysis
In addition to raw data output, BGI offers a range of standard and customized bioinformatics pipelines for your whole genome sequencing project. Reports and output data files are delivered in industry standard file formats: BAM,.xls, and .png.
Standard Analysis
Filtering
Alignment
SNP calling and annotation
SNP database analysis
SNP functionality and conservation prediction
Statistics of SNP distribution on each gene functional element
InDel calling and annotation
InDel database analysis
Statistics of InDel distribution on each gene functional element
Available advanced analysis
Cancer Somatic Mutation analysis
Population genetics analysis
Complex disease analysis
Mendelian disease analysis
De novo mutation analysis for family samples
Customized analysis
Further customization of Bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative
Exome Resources
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.