ChIP-Sequencing is widely used to analyze protein interaction with DNA. It combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify binding sites of DNA-associated proteins, and can be used to precisely map global binding sites for any protein of interest. ChIP sequencing offers higher resolution and more precise and abundant information in comparison with array-based ChIP-chip.
Wide detection range: genome wide protein DNA interaction studies
Cost-effective: less data required for identifying the binding sites in whole genome
Only require low input DNA required: As low as 5ng ChIP-ed DNA for human sample is adequate
Project Workflow
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!
Sequencing Service Specification
The BGI ChIP-Seq Service is performed with the DNBseq sequencing technology, featuring cPAS and DNA Nanoballs (DNB) technology for superior data quality.
Sample Preparation and Services
50bp Single-end sequencing reads
Cloud-based data storage and delivery system
Advanced and Customized bioinformatics analysis
Standard output 20 Million reads per sample
Clean filtered sequencing data delivered in standard FASTQ file format
Turn Around Time
Typical 25 working days from sample QC acceptance to final data delivery
Expedited services are available, contact your local BGI specialist for details
Sample Requirements
We can process your ChIPed DNA with the following general requirements:
DNA Concentration
Minimum Sample Volume
ChIPed DNA Samples
m ≥ 10ng, Concentration ≥ 1 ng/µl
15 μl
Data Analysis
In addition to clean data output, BGI offers a range of standard, advanced and customized bioinformatics pipelines for your ChIP-Seq analysis project. Especially the correlation analysis of RNA-Seq differential expression genes and ChIP-Seq peak related genes. Reports and output data files are delivered in industry standard FASTQ, and Excel file formats with publication-ready tables and figures.
Standard Analysis
Data Filtering and Quality Control
Align Reads to the Reference Genome
Peak Scanning
Peak Annotation
Identify Differential Peaks Between Samples
Differential Peaks Annotation
Advanced Analysis
Motif Analysis (based on 1, 2, 3)
Customized analysis
Statistics of the epigenetic modification level and mRNA expression level of related genes;
The distribution of epigenetic modification level in different gene categories;
Overall connection of epigenetic modification level and the mRNA expression level in different gene categories;
The relationship of the ratio of epigenetic modification and the ratio of mRNA expression in a pair of samples;
Clustering analysis based on epigenetic modification level and mRNA expression level;
Calculate different level of epigenetic modification when mRNA expression level is different;
GO, Pathway analysis, related functional excavation and verification of the genes that differences exist in both epigenetic modification and mRNA expression;
ChIP Resources
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.