ChIP-Seq

ChIP-Seq

Service Description

ChIP-Sequencing is widely used to analyze protein interaction with DNA. It combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify binding sites of DNA-associated proteins, and can be used to precisely map global binding sites for any protein of interest. ChIP sequencing offers higher resolution and more precise and abundant information in comparison with array-based ChIP-chip.

  • Wide detection range: genome wide protein DNA interaction studies
  • Cost-effective: less data required for identifying the binding sites in whole genome
  • Only require low input DNA required: As low as 5ng ChIP-ed DNA for human sample is adequate

Project Workflow

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

  • Sample QC
  • Library QC
  • Sequencing QC
  • Data QC
  • Sample preparation
  • Library preparation
  • Sequencing
  • Raw data output
  • Bioinformatics Analysis

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Sequencing Service Specification

The BGI ChIP-Seq Service is performed with the BGISEQ-500 sequencing system, featuring cPAS and DNA Nanoballs (DNB) technology for superior data quality.

  • Sample Preparation and Services

    Sample Preparation and Services

    • 50bp Single-end sequencing reads
    • Cloud-based data storage and delivery system
    • Advanced and Customized bioinformatics analysis
    • Standard output 20 Million reads per sample
    • Clean filtered sequencing data delivered in standard FASTQ file format
  • Turn Around Time

    Turn Around Time

    • Typical 25 working days from sample QC acceptance to final data delivery
    • Expedited services are available, contact your local BGI specialist for details

Sample Requirements

We can process your ChIPed DNA with the following general requirements:

DNA Concentration

Minimum Sample Volume

ChIPed DNA Samples

m ≥ 10ng, Concentration ≥ 1 ng/µl

15 μl

Data Analysis

In addition to clean data output, BGI offers a range of standard, advanced and customized bioinformatics pipelines for your ChIP-Seq analysis project. Especially the correlation analysis of RNA-Seq differential expression genes and ChIP-Seq peak related genes. Reports and output data files are delivered in industry standard FASTQ, and Excel file formats with publication-ready tables and figures.

Standard Analysis

  • Data Filtering and Quality Control

  • Align Reads to the Reference Genome

  • Peak Scanning

  • Peak Annotation

  • Identify Differential Peaks Between Samples

  • Differential Peaks Annotation

Advanced Analysis

Motif Analysis (based on 1, 2, 3)

Customized analysis

  • Statistics of the epigenetic modification level and mRNA expression level of related genes;

  • The distribution of epigenetic modification level in different gene categories;

  • Overall connection of epigenetic modification level and the mRNA expression level in different gene categories;

  • The relationship of the ratio of epigenetic modification and the ratio of mRNA expression in a pair of samples;

  • Clustering analysis based on epigenetic modification level and mRNA expression level;

  • Calculate different level of epigenetic modification when mRNA expression level is different;

  • GO, Pathway analysis, related functional excavation and verification of the genes that differences exist in both epigenetic modification and mRNA expression;

ChIP Resources

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.