ChIP-Sequencing is widely used to analyze protein interaction with DNA. It combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify binding sites of DNA-associated proteins, and can be used to precisely map global binding sites for any protein of interest. ChIP sequencing offers higher resolution and more precise and abundant information in comparison with array-based ChIP-chip.
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
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The BGI ChIP-Seq Service is performed with the DNBseq sequencing technology, featuring cPAS and DNA Nanoballs (DNB) technology for superior data quality.
We can process your ChIPed DNA with the following general requirements:
ChIPed DNA Samples
m ≥ 10ng, Concentration ≥ 1 ng/µl
15 μl
In addition to clean data output, BGI offers a range of standard, advanced and customized bioinformatics pipelines for your ChIP-Seq analysis project. Especially the correlation analysis of RNA-Seq differential expression genes and ChIP-Seq peak related genes. Reports and output data files are delivered in industry standard FASTQ, and Excel file formats with publication-ready tables and figures.
Data Filtering and Quality Control
Align Reads to the Reference Genome
Peak Scanning
Peak Annotation
Identify Differential Peaks Between Samples
Differential Peaks Annotation
Motif Analysis (based on 1, 2, 3)
Statistics of the epigenetic modification level and mRNA expression level of related genes;
The distribution of epigenetic modification level in different gene categories;
Overall connection of epigenetic modification level and the mRNA expression level in different gene categories;
The relationship of the ratio of epigenetic modification and the ratio of mRNA expression in a pair of samples;
Clustering analysis based on epigenetic modification level and mRNA expression level;
Calculate different level of epigenetic modification when mRNA expression level is different;
GO, Pathway analysis, related functional excavation and verification of the genes that differences exist in both epigenetic modification and mRNA expression;
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.