Target Region Bisulfite Sequencing

Target Region Bisulfite Sequencing

Service Description

Cytosine methylation can significantly modify gene expression and chromatin remodeling. Leveraging the power of next-generation sequencing (NGS), bisulfite sequencing can be done with targeted methods such as amplicon methyl-seq and target enrichment, to provide insight into methylation patterns at a single nucleotide level for specific regions of interest in the human genome as identified, for example, by ENCODE or FANTOM5.

Target region bisulfite sequencing allows researchers to:

  • Reduce sequencing costs compared to whole genome bisulfite sequencing
  • Increase sequence coverage of CpG islands
  • Profile DNA methylations in specific regions of interest in the human genome for studies on development, cell differentiation, cell cycle, DNA repair, genomic imprinting

Project Workflow

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

  • Sample QC
  • Library QC
  • Sequencing QC
  • Data QC
  • Sample preparation
  • Library construction
  • Sequencing
  • Raw data output
  • Bioinformatics analysis

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Sequencing Service Specification

Services are performed using the HiSeq 4000 platform.

  • Turn Around Time

    Turn Around Time

    • Typical 40 working days from sample QC acceptance to filtered raw data availability
    • Expedited services are available, contact your local BGI specialist for details

Sample Requirements

Purity: OD260/280=1.8-2.0, without RNA contamination

DNA concentration

Minimum sample volume

Library Preparation

≥37.5ng/μl

3μg

Data Analysis

Customized analysis

BGI offers extensive analysis options providing unmatched flexibility in delivering reliable data for achieving various research aims ranging from cancer research to understanding basic cellular processes.

Target Region Sequencing Resources

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.