Sample Preparation and Services
- 150bp paired end sequencing
- 250~300bp insert bisulfite treated DNA library
Cytosine methylation can significantly modify gene expression and chromatin remodeling. Leveraging the power of next-generation sequencing (NGS), whole-genome bisulfite sequencing (WGBS) or bisulfite sequencing (Bisulfite-Seq or BS-Seq) is a methodology that uses bisulfite treatment combined with high-throughput sequencing to provide insight into methylation patterns at a single nucleotide level.
WGBS is currently the sole method that can attain both single-base resolution and genome-wide coverage. It has been successfully applied by BGI for elucidation of the methylomes of human cells as well as of many other species such as silkworms, rats, mice, zebra fish, maize and rice.
Whole-genome bisulfite sequencing is considered the ultimate method for methylcytosine analysis as the technique allows researchers to:
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
Whole Genome Bisulfite Sequencing services are performed using the HiSeq platform.
The multi-step process employs bisulfite conversion, PCR amplification and next-generation sequencing to determine the methylation sites across the methylome.
Chemical treatment of DNA with sodium bisulfite converts unmethylated cytosines into uracils. The DNA is then subjected to a PCR assay, where all uracils are converted to thymidines. The resulting DNA amplicons are sequenced at high coverage. The obtained sequencing reads are mapped against a reference genome to enable the identification of the methylated loci in the sample.
We can process your gDNA samples and single-cell applications, with the following general requirements:
Purity: OD260/280 ≥ 1.8 ~ 2.0 without degradation or RNA contamination
≥ 15 ng/μl
≥ 1 μg
BGI offers extensive analysis options providing unmatched flexibility in delivering reliable data for achieving various research aims ranging from cancer research to understanding basic cellular processes.