Sample Preparation and Services
- 100bp paired end sequencing
- Library preparation, including bisulfite treatment
Methylation of DNA at the fifth position in cytosine (5-mC) is a stable epigenetic modification and plays an important role in many biological processes, including gene silencing, suppression of transposable elements, genomic imprinting and X chromosome inactivation. Detection and quantification of methylation are critical to understand gene expression and other processes subjected to epigenetic regulation.
Whole genome bisulfite sequencing (WGBS) is used to detect methylated cytosines by treating the DNA with sodium bisulfite before sequencing. WGBS has become the gold standard for studying genome-wide methylation at single base resolution.
Whole-genome bisulfite sequencing is considered the ultimate method for methylcytosine analysis as the technique allows researchers to:
DNBSEQ™ is an innovative high-throughput sequencing solution, developed by BGI’s Complete Genomics subsidiary in Silicon Valley. The system is powered by combinatorial Probe-Anchor Synthesis (cPAS), linear isothermal Rolling-Circle Replication and DNA Nanoballs (DNBSEQ™) technology, followed by high-resolution digital imaging.
The combination of linear amplification and DNBSEQ™ technology reduces the error rate while enhancing the signal. The size of the DNB is controlled in such a way that only one DNB is bound per active site on the flow cell. This densely patterned array technology provides optimal sequencing accuracy and increases flow cell utilization.
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
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Whole Genome Bisulfite Sequencing services are performed using the DNBseq platform. HiSeq services are available when required.
We can process your gDNA samples, whole blood, cell line, fresh frozen tissue and FFPE samples and single-cell applications, with the following general requirements:
Purity: OD260/280 ≥ 1.8 ~ 2.0 without degradation or RNA contamination
Recommended
Intact genomic DNA ≥ 1μg, Concentration ≥ 50 ng/μl
15μl
Required
Intact genomic DNA ≥ 100ng,
Concentration ≥ 7 ng/μl
15μl
Data Filtering
Alignment
Data Quality Statistics
Methyl-cytosine identification
Differential Methylated Region (DMR) analysis
Statistics of methylation level
BGI offers extensive analysis options providing unmatched flexibility in delivering reliable data for achieving various research aims ranging from cancer research to understanding basic cellular processes.
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
