Whole Genome Bisulfite Sequencing

Whole Genome Bisulfite Sequencing

Service Description

Cytosine methylation can significantly modify gene expression and chromatin remodeling. Leveraging the power of next-generation sequencing (NGS), whole-genome bisulfite sequencing (WGBS) or bisulfite sequencing (Bisulfite-Seq or BS-Seq) is a methodology that uses bisulfite treatment combined with high-throughput sequencing to provide insight into methylation patterns at a single nucleotide level.

WGBS is currently the sole method that can attain both single-base resolution and genome-wide coverage. It has been successfully applied by BGI for elucidation of the methylomes of human cells as well as of many other species such as silkworms, rats, mice, zebra fish, maize and rice.

Whole-genome bisulfite sequencing is considered the ultimate method for methylcytosine analysis as the technique allows researchers to:

  • Simultaneously observe the methylation patterns of all CpG, CHG and CHH sites present in the sample of interest
  • Profile DNA methylations in the entire methylome for studies on development, cell differentiation, cell cycle, DNA repair, genomic imprinting
  • Compare differentially methylated loci from different samples, for example, healthy controls versus patients with cancer
  • Derive correlations between cytosine methylation and transcriptional regulation
  • Investigate inherited methylations in nearly any organism

Project Workflow

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

  • Sample QC
  • Library QC
  • Sequencing QC
  • Data QC
  • Sample preparation
  • Library construction
  • Sequencing
  • Raw data output
  • Bioinformatics analysis

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Sequencing Service Specification

Whole Genome Bisulfite Sequencing services are performed using the HiSeq X-Ten platform.

The multi-step process employs bisulfite conversion, PCR amplification and next-generation sequencing to determine the methylation sites across the methylome.

Chemical treatment of DNA with sodium bisulfite converts unmethylated cytosines into uracils. The DNA is then subjected to a PCR assay, where all uracils are converted to thymidines. The resulting DNA amplicons are sequenced at high coverage. The obtained sequencing reads are mapped against a reference genome to enable the identification of the methylated loci in the sample.

  • Sample Preparation and Services

    Sample Preparation and Services

    • 150bp paired end sequencing
    • 250~300bp insert bisulfite treated DNA library
  • Turn Around Time

    Turn Around Time

    • Typical 40 working days from sample QC acceptance to filtered raw data availability
    • Expedited services are available, contact your local BGI specialist for details

Sample Requirements

We can process your gDNA, Blood, Cell line, Fresh frozen tissue and FFPE samples and
single-cell applications, with the following general requirements:

Purity: OD260/280 ≥ 1.8 ~ 2.0 without degradation or RNA contamination

DNA Concentration

Minimum Sample Volume

Regular Samples

≥ 15 ng/μl

≥ 1 μg

Data Analysis

Customized analysis

BGI offers extensive analysis options providing unmatched flexibility in delivering reliable data for achieving various research aims ranging from cancer research to understanding basic cellular processes.

WGBS Resources

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.