With target region sequencing, a subset of genes or regions of a genome are isolated and sequenced. Targeted approaches using next-generation sequencing (NGS) allow researchers to focus analysis on specific areas of interest.
Compared to broader approaches, such whole genome sequencing, targeted sequencing enables researchers to focus on regions that are most likely to be involved in the phenotype under study, conserving resources and generating a smaller, more manageable data set. Targeted approaches can also deliver much higher coverage levels, allowing identification of variants that are rare and more expensive with whole-genome or Sanger sequencing.
Targeted analysis can include the exome, specific genes of interest or targets within genes.
Mouse Exome Sequencing for Pharmaceutical Research:
BGI has particular experience and expertise providing mouse exome sequencing. Our solution utilizes Agilent SureSelect XT Mouse All Exon Kit, whose 50 Mb capture covers the complete mouse exome and spans over 221,784 exons and 24,306 genes. The content was developed based on the current UCSC mm9 mouse genome build. A BED file with the exon and gene annotation is available on the Agilent Array website. Combined with BGI’s powerful next-generation sequencing platform and bioinformatics analysis capability, we have become one of the most important centers for mouse genome research.
- Highly effective: Designed towards specific species; more effective for capturing the mouse exons
- Wide detection range: Capture both common and rare variants
- Highly cost-effective: Focus on ~1% of the genome where most disease- or trait-related variations are located.
Plant and Animal Targeted Sequencing for Agrigenomics:
The discovery of genomic signatures of evolution and the improvement of plants and animals for agricultural purposes have relied on the identification of SNPs, which are associated with phenotypic differences. In order to identify the genes underlying specific phenotypes, the regions containing the informative SNPs must be fine-mapped. Often, these regions are very large and may contain structural variations that are hard to identify with existing technologies.
BGI offers Single Molecule, Real-Time (SMRT) Sequencing on Pacbio’s Sequel System, which offers a flexible solution for targeting regions of interest, regardless of size, and delivers a comprehensive view of the associated genes. It also delivers the read length and accuracy needed to improve fine mapping and simplify the assembly of large regions.
SMRT Sequencing gives you the ability to:
- Efficiently multiplex large amplicons for rapid screening and identification of variants
- Discover haplotype-specific markers for improved breeding of polyploids
- Accurately reassemble entire multi-megabase regions for the study of gene linkage
- Confirm insertion sites of transgenes and validate gene editing events
- Study informative regions to determine evolutionary or domestication history
- Capture complete genes of varying size for flexible and efficient experimental design in gene surveys