Sequencing result guarenteed
- 150bp paired end sequencing
- Guaranteed ≥ 75% of bases with quality score of ≥Q30
- Raw data, standard and customized data analysis
- Cloud-based data storage and delivery system
BGI is a recognized leader in De Novo Whole Genome Sequencing and has been involved in the sequencing and assembly of 1000s of De Novo genomes and affiliated research published in the world’s leading journals.
De novo sequencing refers to sequencing a novel genome where there is no reference sequence available for alignment.
The process of de novo genome sequencing involves the sequencing of small DNA fragments, and assembling the reads into longer sequences (contigs) and finally ordering the contigs to obtain the entire genome sequence.
With the advent of rapid, low-cost next-generation sequencing (NGS) technology, researchers can now obtain whole genome data for organisms previously considered too low a priority to sequence. The availability of this whole genome data has allowed large-scale genomic studies to be performed that were unimaginable just a few years ago.
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.
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BGI provides various combinations of sequencing platforms, sequencing read-lengths and paired-end and mate-pair library options for De Novo whole genome sequencing applications for animal and plant.
Services are perfomed on either the HiSeq X10, HiSeq4000 or PacBio platforms.
Note: The total quantity of sample required is also determined by the experimental strategy, as well as the type and number of libraries to be constructed.
Short-insert libraries
≥1.5 µg
>20ng/µl
2 kb large-insert libraries
≥20 µg
c>110ng/µl
5 kb-6 kb large-insert libraries
≥20 µg
c>110ng/µl
10 kb large-insert libraries
≥30 µg
c>110ng/µl
20 kb large-insert libraries
≥40 µg
c>106ng/µl
40 kb large-insert libraries
≥50 µg
c>133ng/µl
Genomic DNA
OD260/280= 1.8-2.0
In addition to raw data output, BGI offers a range of standard and customized bioinformatics pipelines for your whole genome sequencing project.
Filtering
K-mer depth distribution analysis and genome size estimation
Genome heterozygous rate estimation
Analysis of GC-depth distribution
Preliminary assembly
Genome Assembly
GC-Depth distribution analysis
GC-Content distribution analysis
Sequencing depth analysis
Evaluation of coverage of autosomal chromosome (BAC or Fosmid sequence should be provided)
Evaluation of coverage of interested genes (EST or transcriptome data should be provided)
Gene family identification (animal: TreeFam; plant: OrthoMCL)
Phylogenetic analysis
Estimation of species divergence time
Whole genome alignment
Segmental duplication analysis (animal: WGD; plant: WGAC)
ncRNA annotation
Gene function annotation
Gene prediction
Repeat annotation
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.