We provide professional, fast and affordable 16S/18S/ITS gene sequencing services for the identification, classification and quantitation of microbes within complex biological mixtures. Contact us today with any questions or for a no obligation quote.
16S and 18S rDNA are hypervariable regions in the 16S or 18S rRNA genes in bacteria and fungus, while ITS (Internal Transcribed Spacer) is the spacer DNA between the small-subunit and large-subunit rRNA genes in bacteria, fungi and archaea.
Sequence comparison of 16S/18S/ITS regions is widely used in taxonomy and molecular phylogenetics because these regions have a high degree of variation even between closely related species, while also offering easy amplification by PCR, even from low quantities of DNA.
We have extensive experience supporting partners across the world with their 16S, 18S rDNA and ITS research needs, while our economies of sequencing scale and proprietary technology ensures extremely efficient pricing.
Sample Requirements
Genomic DNA
Concentration: ≥ 50ng
Volume: ≥ 6ng/μl
PCR Fragment:
Concentration: ≥ 1μg
Volume: ≥ 10ng/μl
Sequencing Standards
• Up to ≥ 80% of bases with a ≥ Q30 quality score, depending on the chosen sequencing strategy
• Recommended sequencing coverage is dependent on the complexity of the sample
Key Service Details
• Typical 45 working days from sample QC acceptance to cleaned raw data availability
• Expedited services are available, contact us for more detail
• Reports and output data files are delivered in industry standard file formats: BAM, .xls, .png and FASTQ data.
BGI's 16S/18S/ITS Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ is a proprietary sequencing technology, first developed by BGI's Complete Genomics subsidiary in Silicon Valley and offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the index hopping rate in DNBSEQ™ platforms to be much lower when compared to that of other platforms.
Contact us with any general enquiries or questions for our sequencing experts, we are here to help.
Access Further Sequencing Resources
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results. Access more resources below for full access to our range of educational resources and sample guides.
Opening a BGI Customer Account enables you to request a quote and order services from us. It also provides access to our range of educational resources including educational webinars providing practical sequencing tips and our complete sample preparation guides.