Sample Preparation and Services
- 50 bp Single End sequencing options available
- Clean data and advanced bioinformatics analysis are available in standard file formats
- Standard and custom bioinformatics data analysis
Small RNAs are a type of non-coding RNA (ncRNA) molecule that are less than 200 nucloetides in length. They are often involved in post-transcriptional regulation through RNA-RNA interactions.
Small RNA sequencing involves first isolating then sequencing small RNA species, such as microRNA (miRNA). Small RNA sequencing captures the complete range of small RNA and miRNA species in a sample, and can detect and quantify thousands of small RNA and miRNA sequences with great sensitivity and dynamic range. It has been applied to discover novel miRNAs and other small noncoding RNAs, to examine the differential expression of all small RNAs in and to characeterize variations with single-base resolution.
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
BGI Small RNA Sequencing services are executed with the BGISEQ-500 sequencing system.
We can process your samples of plant, animal, insect, fungi, prokaryote, plasma, etc. with the following general requirements:
Intact genomic DNA ≥ 1µg, Concentration ≥ 15 ng/ µl
Low Input Samples
Intact genomic DNA ≥ 200 ng, Concentration ≥ 20 ng/ µl
Besides clean data output, BGI offers a range of standard and customized bioinformatics pipelines for your small RNA sequencing project. Reports and output data files are delivered in industry standard file formats: .xls, .png
Summarize the length distribution of small RNA
Analyze common and specific sequences between two samples
Explore small RNA distribution across selected genome
Identify rRNAs, tRNAs, snRNAs, snoRNAs etc.
Identify repeat associated small RNAs
Identify small RNA reads which could align to exon/intron
Identify known miRNAs by aligning to designated part of miRBase
Analyze the expression pattern of known miRNAs
Annotate small RNAs into several categories based on priority
Predict novel miRNAs and their secondary structures by Mireap and miRDeep from unannotated small RNAs
Family analysis of known miRNAs
Target genes prediction of novel known miRNA and miRNA
GO annotation and KEGG pathway analysis of known miRNA and novel miRNA Target genes
Base editing analysis of known miRNA
Differential expression analysis of known miRNA
Differential expression analysis of novel miRNA
Target genes prediction of differential miRNA
GO annotation and KEGG pathway analysis of differential miRNA Target genes
Further customization of Bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative