Small RNA Sequencing

Small RNA Sequencing

Service Description

Small RNAs are a type of non-coding RNA (ncRNA) molecules that are less than 200nt in length. They are often involved in gene silencing and post- transcriptional regulation of gene expression. Small RNA sequencing is used to discover novel small RNAs, examine the dierential expression of all small RNAs and to characterize variations with single-base resolution.

Project Workflow

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

  • Sample QC
  • Library QC
  • Sequencing QC
  • Data QC
  • Sample Preparation
  • Library Preparation
  • Sequencing
  • Raw Data Output
  • Bioinformatics Analysis

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Sequencing Service Specification

BGI Small RNA Sequencing services are executed with the DNBseq sequencing technology.

  • Sample Preparation and Services

    Sample Preparation and Services

    • 50bp single-end sequencing reads
    • Standard output 20 Million reads per sample
    • Clean data and bioinformatics analysis are available in standard file formats
    • Standard and customized bioinformatics data analysis are available
    • Cloud-based data storage and delivery system
  • Sequencing Quality Standard

    Sequencing Quality Standard

    • Q20≥90%
    • Adaptor and null rate≥10%
    • Small RNA tag rate≥20%
    • Clean reads: no less than quantity the contract needed
  • Turn Around Time

    Turn Around Time

    • Typical 30 working days from sample QC acceptance to data delivery
    • Expedited services are available, contact your local BGI specialist for details

Sample Requirements

We can process your sample of human, plant, animal, and microbial samples, with the following general requirements:

AMOUNT AND CONCENTRATION

MINIMUM VOLUME

QUANTITATIVE RESULT

Total RNA Samples

Total RNA≥1μg, Concentration≥15 ng/μl

15 μl

RIN≥7.5;28S/18S≥1.3

Data Analysis

In addition to clean data output, BGI offers a range of standard and customized bioinformatics pipelines for your small RNA sequencing project.
Reports and output data files are delivered in industry standard FASTQ, and Excel file formats with publication-ready tables and figures.

STANDARD BIOINFORMATICS ANALYSIS

  • Data filtering

  • Length distribution of small RNAs

  • Analysis of common and specific sequences between two samples

  • Small RNAs distribution across selected genome

  • Identification of rRNAs, tRNAs, snRNAs, snoRNAs, etc.

  • Identification of repeat associated small RNAs

  • Identification of small RNA sequences which could align to exon/intron

  • Identification of known miRNAs by aligning to designated part of miRBase

  • Analysis of the expression pattern of known miRNAs

  • Classification of small RNAs into several categories based on customer preference

  • Prediction of novel miRNAs and their secondary structures by Mireap and miRDeep from unannotated small RNAs

  • Family analysis of known miRNAs

CUSTOMIZED ANALYSIS

Further customization of Bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative

Small RNA Resources

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.