Small RNA Sequencing

Small RNA Sequencing

Service Description

Small RNAs are a type of non-coding RNA (ncRNA) molecule that are less than 200 nucloetides in length. They are often involved in post-transcriptional regulation through RNA–-RNA interactions.

Small RNA sequencing involves first isolating then sequencing small RNA species, such as microRNA (miRNA). Small RNA sequencing captures the complete range of small RNA and miRNA species in a sample, and can detect and quantify thousands of small RNA and miRNA sequences with great sensitivity and dynamic range. It has been applied to discover novel miRNAs and other small noncoding RNAs, to examine the differential expression of all small RNAs in and to characeterize variations with single-base resolution.

Project Workflow

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

  • Sample QC
  • Library QC
  • Sequencing QC
  • Data QC
  • Sample Preparation
  • Library Preparation
  • Sequencing
  • Raw Data Output
  • Bioinformatics Analysis

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Sequencing Service Specification

BGI Small RNA Sequencing services are executed with the DNBseq sequencing technology.

  • Sample Preparation and Services

    Sample Preparation and Services

    • 50 bp Single End sequencing options available
    • Clean data and advanced bioinformatics analysis are available in standard file formats
    • Standard and custom bioinformatics data analysis
  • Sequencing Quality Standard

    Sequencing Quality Standard

    • Q20≥90%
    • Adaptor and null rate≥10%
    • Small RNA tag rate≥20%
    • Clean reads: no less than quantity the contract needed
  • Turn Around Time

    Turn Around Time

    • Typical 30 working days from sample QC acceptance to filtered raw data availability
    • Expedited services are available, contact your local BGI specialist for details

Sample Requirements

We can process your samples of plant, animal, insect, fungi, prokaryote, plasma, etc. with the following general requirements:

DNA Amount and Concentration

Minimum Sample Volume

Regular Samples

Intact genomic DNA ≥ 1µg, Concentration ≥ 15 ng/ µl

15 μl

Low Input Samples

Intact genomic DNA ≥ 200 ng, Concentration ≥ 20 ng/ µl

15 μl

Data Analysis

Besides clean data output, BGI offers a range of standard and customized bioinformatics pipelines for your small RNA sequencing project. Reports and output data files are delivered in industry standard file formats: .xls, .png

Standard Analysis

  • Filtering

  • Summarize the length distribution of small RNA

  • Analyze common and specific sequences between two samples

  • Explore small RNA distribution across selected genome

  • Identify rRNAs, tRNAs, snRNAs, snoRNAs etc.

  • Identify repeat associated small RNAs

  • Identify small RNA reads which could align to exon/intron

  • Identify known miRNAs by aligning to designated part of miRBase

  • Analyze the expression pattern of known miRNAs

  • Annotate small RNAs into several categories based on priority

  • Predict novel miRNAs and their secondary structures by Mireap and miRDeep from unannotated small RNAs

  • Family analysis of known miRNAs

Available advanced analysis

  • Target genes prediction of novel known miRNA and miRNA

  • GO annotation and KEGG pathway analysis of known miRNA and novel miRNA Target genes

  • Base editing analysis of known miRNA

  • Differential expression analysis of known miRNA

  • Differential expression analysis of novel miRNA

  • Target genes prediction of differential miRNA

  • GO annotation and KEGG pathway analysis of differential miRNA Target genes

  • piRNA annotation

  • snoRNA annotation

Customized analysis

Further customization of Bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative

Small RNA Resources

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.