Transcriptome Sequencing

Transcriptome Sequencing

Service Description

Transcriptome sequencing is used to reveal the presence, quantity and structure of RNA in a biological sample under specific conditions. Compared to hybridization-based RNA quantification methods such as microarray analysis, sequencing-based transcriptome detection can quantify gene expression with low background, high accuracy and high levels of reproducibility within a large dynamic range. In addition, transcriptome sequencing does not require an existing genome sequence and can detect mutations, splice variants and fusion genes that can not be detected by microarrays.

 

Unique DNBseq™ Technology

BGI Transcriptome Sequencing services are executed with the DNBseq sequencing technology, for great sequencing data at the lowest cost in the industry.

DNBseq is BGI’s proprietary sequencing platform, developed by our Complete Genomics subsidiary in Silicon Valley.

Give our DNBseq Transcriptome Sequencing services a try and find out why so many trust their valuable research samples to the world-class sequencing experience of BGI.

Ask your BGI representative about our current introductory pricing for Transcriptome Sequencing with the BGI’s own DNBseq sequencing technology.

Applications

Project Workflow

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

  • Sample QC
  • Library QC
  • Sequencing QC
  • Data QC
  • Sample Preparation
  • Transcriptome Library Preparation
  • Sequencing
  • Raw Data Output
  • Bioinformatics Analysis

How to Order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Sequencing Service Specification

BGI Transcriptome Sequencing services are executed with the DNBseq sequencing technology with the benefit of lower cost and fast turnaround time.

  • Sample Preparation and Services

    Sample Preparation and Services

    • Multiple choices for mRNA enrichment and rRNA removal kits
    • Stranded and non-stranded sequencing is available
    • 100bp paired-end sequencing
    • ≥30 Million reads per sample recommended
    • Raw data and bioinformatics analysis are available in standard file formats
    • Advanced and custom bioinformatics data analysis
    • Cloud-based data storage and delivery system
  • Sequencing Quality Standard

    Sequencing Quality Standard

    • Guaranteed ≥80% of bases with quality score of ≥Q30
  • Turnaround Time

    Turnaround Time

    • Typical 30 working days from sample QC acceptance to filtered raw data availability
    • Expedited services are available, contact your local BGI specialist for details

Sample Requirements

We can process your total RNA, blood, cell line, FFPE, fresh frozen tissues and single cell samples from a variety of species, with the following general requirements.

OD:
OD260/280 ≥1.8
OD260/230 ≥1.8

SPECIES

RNA AMOUNT AND CONCENTRATION

RIN VALUE

Human/Animal

Total RNA ≥200ng,
Concentration ≥20ng/μl

RIN≥7.0
28S/18S≥1.0

Fungi

Total RNA ≥1μg,
Concentration ≥40ng/μ

RIN≥6.5
28S/18S≥1.0

Plant

Total RNA ≥1μg,
Concentration ≥40ng/μl

RIN≥6.5
28S/18S≥1.0

Insects

Total RNA ≥1μg,
Concentration ≥40ng/μl

NA

Prokaryotes

Total RNA ≥1μg,
Concentration ≥40ng/μl

RIN≥7.0
23S/16S≥1.0

Data Analysis

In addition to raw data output, BGI offers a range of standard and customized bioinformatics pipelines for your transcriptome sequencing project. Reports and output data files are delivered in industry standard file formats: FASTQ, BAM and Excel.

Standard Analysis (With Reference)

  • Quantitative expression profiles

  • Alternative splicing analysis

  • Fusion gene analysis

  • SNP and Indel detection

  • Time series analysis

  • Gene ontology analysis

  • Pathway enrichment analysis

  • Hierarchical clustering analysis

  • Protein-Protein Interaction (PPI) analysis

  • Fungal pathogenic gene annotation (For fungi)

  • Plant disease resistance genes annotation (For plant)

Customized Analysis

Further customization of Bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative.