Proteome Profiling


Project Workflow

  • Protein extraction
  • Protein digestion
  • Isobaric labeling
  • HPLC fractionation
  • LC-MS/MS (DDA)
  • Data analysis

Mass Spectrometry Service Specification

Isobaric Label Quantitative Proteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF/HF-X).
  • Sample Preparation and Services

    • The QC of enzymatic hydrolysis and labeling steps are strictly controlled by LC-MS DDA analysis
    • 1h/fraction; 20 fractions for eukaryotes;10 fractions for prokaryotes
  • Quality Standard

    • Summary includes all methods and data analysis
    • Reports provided in Excel or PDF format, RAW files available upon request
  • Turn Around Time

    • Typical 4-5 weeks from sample QC acceptance to data report delivery
AnimalCommon animal tissues: animal internal organs (heart, liver, spleen, lung, kidney), skin, muscle, brain, etc≥ 10 mg
AnimalMollusks (Toxoplasma, Schistosomiasis, Drosophila, Acarid, Plutella xylostella, Laodelphax, Cestode, Cicada, Hematodinium, etc.)≥ 10 mg
CellSuspended cells, adherent cells≥ 1×10^7
CellCell culture supernatant≥ 10 mg
ExosomeExosome isolated by customer≥ 50 µg, ≥ 0.5 µg/µL
FluidPlasma, serum (remove highly-abundant protein)≥ 200 µL
FluidPlasma, serum (with highly-abundant protein)≥ 10 µL
Fluid Amniotic fluid, cerebrospinal fluid, semen, etc. (remove highly-abundant protein)≥ 1 mL
FluidAmniotic fluid, cerebrospinal fluid, semen, etc. (with highly-abundant protein)≥ 200 µL
FluidSaliva, milk≥ 200 µL
FluidUrine≥ 30 mL
PlantTear≥ 15 µL
PlantTwigs of plants (leaf buds, tender leaves), algae≥ 300 mg
PlantOld leaves, roots, stems, bark of plants≥ 1 g
PlantPlant buds, pollen≥ 100 mg
PlantPlant seeds (rice/wheat seeds, etc.), fruits (apples, peaches, pears)≥ 1 g
MicroorganismProkaryotic bacteria (E. coli, Staphylococcus aureus, etc.), fungi (yeast, etc.)Thallus ≥ 50 mg cells ≥ 5×10^6
Protein solutionComplex protein solution, protein powder≥ 100 µg, ≥ 0.5 µg/µL
OthersFeces≥ 300 mg

Data Analysis

  • Data analysis and validation performed with Mascot
  • Protein identification and quantification list
  • Differential proteins data statistics and volcano plot
  • Principal component analysis (PCA) and cluster analysis
  • Protein GO/COG/KOG/Pathway annotation
  • GO/COG/KOG enrichment analysis of differential proteins
  • Protein-protein interaction analysis
  • Protein subcellular localization analysis
  • Repeatability analysis